A pathway of signals regulating effector and initiator caspases in the developing Drosophila eye

被引:0
作者
Yu, SY
Yoo, SJ
Yang, LH
Zapata, C
Srinivasan, A
Hay, BA
Baker, NE
机构
[1] Yeshiva Univ Albert Einstein Coll Med, Dept Mol Genet, Bronx, NY 10461 USA
[2] CALTECH, Div Biol, Pasadena, CA 91125 USA
[3] IDUN Pharmaceut, La Jolla, CA 92037 USA
来源
DEVELOPMENT | 2002年 / 129卷 / 13期
关键词
Drosophila eye; caspase; Drice; Drone; Notch; EGF receptor; hid; IAP; apoptosis;
D O I
暂无
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Regulated cell death and survival play important roles in neural development. Extracellular signals are presumed to regulate seven apparent caspases to determine the final structure of the nervous system. In the eye, the EGF receptor, Notch, and intact primary pigment and cone cells have been implicated in survival or death signals. An antibody raised against a peptide from human caspase 3 was used to investigate how extracellular signals controlled spatial patterning of cell death. The antibody crossreacted specifically with dying Drosophila cells and labelled the activated effector caspase Drice. It was found that the initiator caspase Dronc and the proapoptotic gene head involution defective were important for activation in vivo. Drone may play roles in dying cells in addition to activating downstream effector caspases. Epistasis experiments ordered EGF receptor, Notch, and primary pigment and cone cells into a single pathway that affected caspase activity in pupal retina through hid and Inhibitor of Apoptosis Proteins. None of these extracellular signals appeared to act by initiating caspase activation independently of hid. Taken together, these findings indicate that in eye development spatial regulation of cell death and survival is integrated through a single intracellular pathway.
引用
收藏
页码:3269 / 3278
页数:10
相关论文
共 59 条
  • [1] Spodoptera frugiperda caspase-1, a novel insect death protease that cleaves the nuclear immunophilin FKBP46, is the target of the baculovirus antiapoptotic protein p35
    Ahmad, M
    Srinivasula, SM
    Wang, LJ
    Litwack, G
    FernandesAlnemri, T
    Alnemri, ES
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (03) : 1421 - 1424
  • [2] Apoptotic molecular machinery: Vastly increased complexity in vertebrates revealed by genome comparisons
    Aravind, L
    Dixit, VM
    Koonin, EV
    [J]. SCIENCE, 2001, 291 (5507) : 1279 - +
  • [3] The EGF receptor defines domains of cell cycle progression and survival to regulate cell number in the developing Drosophila eye
    Baker, NE
    Yu, SY
    [J]. CELL, 2001, 104 (05) : 699 - 708
  • [4] Cell proliferation, survival, and death in the Drosophila eye
    Baker, NE
    [J]. SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 2001, 12 (06) : 499 - 507
  • [5] ELLIPSE MUTATIONS IN THE DROSOPHILA HOMOLOG OF THE EGF RECEPTOR AFFECT PATTERN-FORMATION, CELL-DIVISION, AND CELL-DEATH IN EYE IMAGINAL DISKS
    BAKER, NE
    RUBIN, GM
    [J]. DEVELOPMENTAL BIOLOGY, 1992, 150 (02) : 381 - 396
  • [6] The Drosophila gene hid is a direct molecular target of Ras-dependent survival signaling
    Bergmann, A
    Agapite, J
    McCall, K
    Steller, H
    [J]. CELL, 1998, 95 (03) : 331 - 341
  • [7] The mitochondrial apoptotic pathway is activated by serum and glucose deprivation in cardiac myocytes
    Bialik, S
    Cryns, VL
    Drincic, A
    Miyata, S
    Wollowick, AL
    Srinivasan, A
    Kitsis, RN
    [J]. CIRCULATION RESEARCH, 1999, 85 (05) : 403 - 414
  • [8] Biochemical pathways of caspase activation during apoptosis
    Budihardjo, I
    Oliver, H
    Lutter, M
    Luo, X
    Wang, XD
    [J]. ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 1999, 15 : 269 - 290
  • [9] INHIBITION OF ICE FAMILY PROTEASES BY BACULOVIRUS ANTIAPOPTOTIC PROTEIN P35
    BUMP, NJ
    HACKETT, M
    HUGUNIN, M
    SESHAGIRI, S
    BRADY, K
    CHEN, P
    FERENZ, C
    FRANKLIN, S
    GHAYUR, T
    LI, P
    LICARI, P
    MANKOVICH, J
    SHI, LF
    GREENBERG, AH
    MILLER, LK
    WONG, WW
    [J]. SCIENCE, 1995, 269 (5232) : 1885 - 1888
  • [10] Eggs to die for:: cell death during Drosophila oogenesis
    Buszczak, M
    Cooley, L
    [J]. CELL DEATH AND DIFFERENTIATION, 2000, 7 (11) : 1071 - 1074