Boronic Acid vs. Folic Acid: A Comparison of the bio-recognition performances by Impedimetric Cytosensors based on Ferrocene cored dendrimer

被引:23
作者
Dervisevic, Muamer [1 ]
Senel, Mehmet [1 ]
Sagir, Tugba [2 ]
Isik, Sevim [1 ]
机构
[1] EMC Technology Inc, Biotechnol Res Lab, ARGEM Bldg,Technocity,Aucilar, TR-34320 Istanbul, Turkey
[2] Orta Mah, Sunay Sok 1, TR-34880 Istanbul, Turkey
关键词
Folic acid; Boronic acid; Cancer cells; Electrochemical impedance; Ferrocene; Cytosensor; SENSITIVE DETECTION; CANCER-CELLS; SIALIC-ACID; BIOSENSOR; DIAGNOSIS; DEVICE; BLOOD;
D O I
10.1016/j.bios.2017.01.030
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
A comparative study is reported where folic acid (FA) and boronic acid (BA) based cytosensors and their analytical performances in cancer cell detection were analyzed by using electrochemical impedance spectroscopy (EIS) method. Cytosensors were fabricated using self -assembled monolayer principle by modifying Au electrode with cysteamine (Cys) and immobilization of ferrocene cored polyamidiamine dendrimers second generation (Fc-PAMAM (G2)), after which electrodes were modified with FA and BA. Au/Fc-PAMAM(G2)/FA and Au/Fc-PAMAM(G2)/BA based cytosensors showed extremely good analytical performances in cancer cell detection with linear range of 1 x10(2) to 1 x10(6) cells ml(-1), detection limit of 20 cells ml(-1) with incubation time of 20 min for FA based electrode, and for BA based electrode detection limit was 28 cells ml(-1) with incubation time of 10 min. Next to excellent analytical performances, cytosensors showed high selectivity towards cancer cells which was demonstrated in selectivity study using human embryonic kidney 293 cells (HEK 293) as normal cells and Au/Fc-PAMAM(G2)/FA electrode showed two times better selectivity than BA modified electrode. These cytosensors are promising for future applications in cancer cell diagnosis.
引用
收藏
页码:680 / 686
页数:7
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