Bacillus megaterium strains derived from water and soil exhibit differential responses to the herbicide mesotrione

被引：21

作者：

Dobrzanski, Tatiane ^{[1
]}

Gravina, Fernanda ^{[1
]}

Steckling, Bruna ^{[1
]}

Olchanheski, Luiz R. ^{[2
]}

Sprenger, Ricardo F. ^{[3
]}

Espirito Santo, Bruno C. ^{[4
]}

Galvao, Carolina W. ^{[5
]}

Reche, Pericles M. ^{[6
]}

Prestes, Rosilene A. ^{[7
]}

Pileggi, Sonia A. V. ^{[1
]}

Campos, Francinete R. ^{[8
]}

Azevedo, Ricardo A. ^{[9
]}

Sadowsky, Michael J. ^{[10
,11
]}

Beltrame, Flavio L. ^{[12
]}

Pileggi, Marcos ^{[1
]}

机构：

[1] Univ Estadual Ponta Grossa, Dept Biol Estrutural Mol & Genet, Setor Ciencias Biol & Saude, Lab Microbiol Ambiental, Ponta Grossa, Parana, Brazil

[2] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Lab Biol Mol & Ecol Microbiana, Sao Paulo, SP, Brazil

[3] Univ Fed Sao Carlos, Dept Quim, Separare Nucleo Cromatog, Sao Carlos, SP, Brazil

[4] Univ Estadual Maringe, Dept Biotecnol Genet & Biol Celular, Lab Biotecnol Microbiana, Maringa, Parana, Brazil

[5] Univ Estadual Ponta Grossa, Dept Biol Estrutural Mol & Genet, Setor Ciencias Biol & Saude, Lab Biol Mol Microbiana, Ponta Grossa, Parana, Brazil

[6] Univ Estadual Ponta Grossa, Dept Enfermagem & Saude Publ, Setor Ciencias Biol & Saude, Lab Pesquisa Recursos Hidricos, Ponta Grossa, Parana, Brazil

[7] Univ Tecnol Fed Parana, UTFPR, Dept Acad, Campus Ponta Grossa, Ponta Grossa, Parana, Brazil

[8] Univ Fed Parana, Dept Farm, Lab Biociencias & Espectrometria Massas, Curitiba, Parana, Brazil

[9] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Genet, Piracicaba, SP, Brazil

[10] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA

[11] Univ Minnesota, Biotechnol Inst, St Paul, MN 55108 USA

[12] Univ Estadual Ponta Grossa, Dept Ciencias Farmaceut, Setor Ciencias Biol & Saude, Lab Fitoterapia Tecnol & Quin Prod Nat, Ponta Grossa, Parana, Brazil

来源：

PLOS ONE | 2018年 / 13卷 / 04期

基金：

巴西圣保罗研究基金会;

关键词：

OXIDATIVE STRESS; ESCHERICHIA-COLI; ANTIOXIDANT RESPONSES; TOXICITY ASSESSMENT; DEGRADING BACTERIA; HYDROGEN-PEROXIDE; DEGRADATION; MEMBRANE; ENZYMES; COMMUNITIES;

D O I：

10.1371/journal.pone.0196166

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The intense use of herbicides for weed control in agriculture causes selection pressure on soil microbiota and water ecosystems, possibly resulting in changes to microbial processes, such as biogeochemical cycles. These xenobiotics may increase the production of reactive oxygen species and consequently affect the survival of microorganisms, which need to develop strategies to adapt to these conditions and maintain their ecological functionality. This study analyzed the adaptive responses of bacterial isolates belonging to the same species, originating from two different environments (water and soil), and subjected to selection pressure by herbicides. The effects of herbicide Callisto and its active ingredient, mesotrione, induced different adaptation strategies on the cellular, enzymatic, and structural systems of two Bacillus megaterium isolates obtained from these environments. The lipid saturation patterns observed may have affected membrane permeability in response to this herbicide. Moreover, this may have led to different levels of responses involving superoxide dismutase and catalase activities, and enzyme polymorphisms. Due to these response systems, the strain isolated from water exhibited higher growth rates than did the soil strain, in evaluations made in oligotrophic culture media, which would be more like that found in semi-pristine aquatic environments. The influence of the intracellular oxidizing environments, which changed the mode of degradation of mesotrione in our experimental model and produced different metabolites, can also be observed in soil and water at sites related to agriculture. Since the different metabolites may present different levels of toxicity, we suggest that this fact should be considered in studies on the fate of agrochemicals in different environments.

引用

页数：24

共 70 条

[1]

[Anonymous], PROCESS BIOCH

[2] Response of antioxidant enzymes to transfer from elevated carbon dioxide to air and ozone fumigation, in the leaves and roots of wild-type and a catalase-deficient mutant of barley [J].