Comparison of Heat Inactivation and Cell Disruption Protocols for Identification of Mycobacteria from Solid Culture Media by Use of Vitek Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

被引：42
作者：
Machen, Alexandra [1 ]
Kobayashi, Miwako [1 ]
Connelly, Mary Robin [2 ]
Wang, Yun F. [1 ,3 ]
机构：
[1] Emory Univ, Sch Med, Atlanta, GA 30322 USA
[2] Georgia Publ Hlth Lab, Atlanta, GA USA
[3] Grady Mem Hosp, Atlanta, GA USA
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2013年 / 51卷 / 12期
关键词：
MALDI-TOF MS; RAPID IDENTIFICATION; INTACT MYCOBACTERIA; DISCRIMINATION; MICROORGANISMS;
D O I：
10.1128/JCM.02612-13
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Two novel protocols for inactivation and extraction were developed and used to identify 107 Mycobacterium clinical isolates, including Mycobacterium tuberculosis complex, from solid cultures using Vitek matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. The protocol using heat inactivation with sonication and cell disruption with glass beads resulted in 82.2% and 88.8% species and genus level identifications, respectively.
引用
收藏
页码：4226 / 4229
页数：4
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