Comparison of Heat Inactivation and Cell Disruption Protocols for Identification of Mycobacteria from Solid Culture Media by Use of Vitek Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

被引：44

作者：

Machen, Alexandra ^{[1
]}

Kobayashi, Miwako ^{[1
]}

Connelly, Mary Robin ^{[2
]}

Wang, Yun F. ^{[1
,3
]}

机构：

[1] Emory Univ, Sch Med, Atlanta, GA 30322 USA

[2] Georgia Publ Hlth Lab, Atlanta, GA USA

[3] Grady Mem Hosp, Atlanta, GA USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2013年 / 51卷 / 12期

关键词：

MALDI-TOF MS; RAPID IDENTIFICATION; INTACT MYCOBACTERIA; DISCRIMINATION; MICROORGANISMS;

D O I：

10.1128/JCM.02612-13

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two novel protocols for inactivation and extraction were developed and used to identify 107 Mycobacterium clinical isolates, including Mycobacterium tuberculosis complex, from solid cultures using Vitek matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. The protocol using heat inactivation with sonication and cell disruption with glass beads resulted in 82.2% and 88.8% species and genus level identifications, respectively.

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收藏

页码：4226 / 4229

页数：4

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