Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions

被引：70

作者：

Lee, Sang Choel ^{[2
]}

Han, Seung Hyeok ^{[1
]}

Li, Jin Ji ^{[1
,3
]}

Lee, Sun Ha ^{[1
]}

Jung, Dong-Sub ^{[1
]}

Kwak, Seung-Jae ^{[1
]}

Kim, Seung Hye ^{[1
]}

Kim, Dong Ki ^{[1
]}

Yoo, Tae-Hyun ^{[1
]}

Kim, Jin Hyun ^{[4
]}

Chang, Se-Ho ^{[5
]}

Han, Dae Suk ^{[1
]}

Kang, Shin-Wook ^{[1
]}

机构：

[1] Yonsei Univ, Coll Med, Dept Internal Med, Brain Korea Project Med Sci 21, Seoul 120752, South Korea

[2] Kwandong Univ, Coll Med, Dept Internal Med, Goyang, Gyeonggi Do, South Korea

[3] YanBian Univ, Coll Med, Dept Internal Med, Affiliated Hosp,Nephrol & Dialysis Unit, Yanji, Jilin, Peoples R China

[4] Gyeongsang Natl Univ, Coll Med, Dept Anat & Neurobiol, Gyeongnam, South Korea

[5] Gyeongsang Natl Univ, Coll Med, Dept Internal Med, Gyeongnam, South Korea

来源：

KIDNEY INTERNATIONAL | 2009年 / 76卷 / 08期

关键词：

apoptosis; diabetic nephropathy; heme oxygenase-1; high glucose; podocyte; RENAL INJURY; DIFFERENTIAL EXPRESSION; OXIDATIVE STRESS; MESSENGER-RNA; GROWTH-FACTOR; KIDNEY; GENE; ISCHEMIA; REPERFUSION; PATHWAYS;

D O I：

10.1038/ki.2009.286

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose-treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose-treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions. Kidney International (2009) 76, 838-848; doi:10.1038/ki.2009.286; published online 5 August 2009

引用

页码：838 / 848

页数：11

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