C-terminal truncation of WT1 delays but does not abolish hematopoiesis in embryoid bodies

被引：4

作者：

Wagner, KJ

Patek, CE

Cunningham, A

Taylor, AH

Hooper, ML

Ansell, JD

机构：

[1] Univ Edinburgh, Western Gen Hosp, Sir Alastair Currie Canc Res UK Labs, Mol Med Ctr, Edinburgh EH4 2XU, Midlothian, Scotland

[2] Univ Edinburgh, Western Gen Hosp, John Hughes Bennett Lab, Edinburgh EH4 2XU, Midlothian, Scotland

来源：

BLOOD CELLS MOLECULES AND DISEASES | 2002年 / 28卷 / 03期

关键词：

D O I：

10.1006/bcmd.2002.0529

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The effect of mutations that truncate the WT1 protein on in vitro hematopoietic differentiation from embryonal stem cells has been examined by CFU-A assay, o-dianisidine staining for heme, and RT-PCR analysis of the expression of fetal and adult globins. In two independently isolated ES cell lines the mutations delay but do not abolish hematopoiesis. Analysis of replated CFU-A colonies indicates that the delay occurs prior to the formation of hematopoietic stem cells. The results demonstrate a role for WT1 at the onset of hematopoiesis. (C) 2002 Elsevier Science (USA).

引用

页码：428 / 435

页数：8

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