Severe Acute Respiratory Syndrome Coronavirus 2 Nucleocapsid Antigen in Urine of Hospitalized Patients With Coronavirus Disease 2019

被引:7
作者
Veyrenche, Nicolas [1 ,2 ]
Pisoni, Amandine [1 ,2 ]
Debiesse, Segolene [1 ,2 ]
Bollore, Karine [1 ,2 ]
Bedin, Anne-Sophie [1 ,2 ]
Makinson, Alain [3 ,4 ]
Niel, Clemence [5 ]
Alcocer-Cordellat, Carmen [5 ]
Mondain, Anne-Marie [5 ]
Le Moing, Vincent [3 ,4 ]
Van de Perre, Philippe [1 ,2 ]
Tuaillon, Edouard [1 ,2 ]
机构
[1] Univ Montpellier, Univ Antilles, EFS, INSERM,Pathogenesis & Control Chron & Emerging In, Montpellier, France
[2] CHU Montpellier, 371 Av Doyen Gaston Giraud, F-34090 Montpellier, France
[3] Univ Hosp, Trop & Infect Dis, Montpellier, France
[4] IRD, INSERM U1175 IRD UMI 233, Montpellier, France
[5] Montpellier Univ Hosp, Montpellier, France
关键词
COVID-19; SARS-CoV-2; Nucleocapsid; Urine; Severity; SARS-COV-2;
D O I
10.1093/infdis/jiac073
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid antigen (N-Ag) can be detected in the blood of patients with coronavirus disease 2019 (COVID-19). We used a highly sensitive and specific assay to explore the presence of N-Ag in urine during the course of COVID-19 and its relationship with the severity of disease. Methods We studied urinary and plasma N-Ag using a highly sensitive immunoassay in 82 patients with SARS-CoV-2 infection proved by polymerase chain reaction. Results In the first and second weeks of COVID-19, hospitalized patients tested positive for urinary N-Ag (81.25% and 71.79%, respectively) and plasma N-Ag (93.75% and 94.87%, respectively). High urinary N-Ag levels were associated with the absence of SARS-CoV-2 nucleocapsid antibodies, admission in intensive care units, high C-reactive protein levels, lymphopenia, eosinopenia, and high lactate dehydrogenase levels. Higher accuracy was observed for urinary N-Ag as a predictor of severe COVID-19 than for plasma N-Ag. Conclusions Our study demonstrates that N-Ag is present in the urine of patients hospitalized in the early phase of COVID-19. As a direct marker of SARS-CoV-2, urinary N-Ag reflects the dissemination of viral compounds in the body. Urinary N-Ag may be a useful marker for disease severity in SARS-CoV-2 infections. Mechanisms leading to severe coronavirus disease 2019 (COVID-19) remain poorly understood. We demonstrate that severe acute respiratory syndrome coronavirus 2 nucleocapsid protein is present in high concentrations in the urine of hospitalized patients with COVID-19, concentrations associated with COVID-19 severity.
引用
收藏
页码:812 / 821
页数:10
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