Genetic Detection and Characterization of Lujo Virus, a New Hemorrhagic Fever-Associated Arenavirus from Southern Africa

被引：310

作者：

Briese, Thomas ^{[1
]}

Paweska, Janusz T. ^{[2
]}

McMullan, Laura K. ^{[3
]}

Hutchison, Stephen K.

Street, Craig ^{[1
]}

Palacios, Gustavo ^{[1
]}

Khristova, Marina L. ^{[4
]}

Weyer, Jacqueline ^{[2
]}

Swanepoel, Robert ^{[2
]}

Egholm, Michael

Nichol, Stuart T. ^{[3
]}

Lipkin, W. Ian ^{[1
]}

机构：

[1] Columbia Univ, Mailman Sch Publ Hlth, Ctr Infect & Immun, New York, NY 10027 USA

[2] Natl Inst Communicable Dis, Special Pathogens Unit, Natl Hlth Lab Serv, Johannesburg, South Africa

[3] Ctr Dis Control & Prevent, Div Viral & Rickettsial Dis, Special Pathogens Branch, Atlanta, GA USA

[4] Ctr Dis Control & Prevent, Biotechnol Core Facil Branch, Atlanta, GA USA

来源：

PLOS PATHOGENS | 2009年 / 5卷 / 05期

基金：

美国国家卫生研究院;

关键词：

LYMPHOCYTIC CHORIOMENINGITIS VIRUS; WHITEWATER-ARROYO VIRUS; LASSA-VIRUS; ENVELOPE GLYCOPROTEIN; SIGNAL PEPTIDE; AMERICAN ARENAVIRUS; SUBTILASE SKI-1/S1P; ALPHA-DYSTROGLYCAN; WORLD ARENAVIRUSES; FINGER PROTEIN;

D O I：

10.1371/journal.ppat.1000455

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Lujo virus (LUJV), a new member of the family Arenaviridae and the first hemorrhagic fever-associated arenavirus from the Old World discovered in three decades, was isolated in South Africa during an outbreak of human disease characterized by nosocomial transmission and an unprecedented high case fatality rate of 80% (4/5 cases). Unbiased pyrosequencing of RNA extracts from serum and tissues of outbreak victims enabled identification and detailed phylogenetic characterization within 72 hours of sample receipt. Full genome analyses of LUJV showed it to be unique and branching off the ancestral node of the Old World arenaviruses. The virus G1 glycoprotein sequence was highly diverse and almost equidistant from that of other Old World and New World arenaviruses, consistent with a potential distinctive receptor tropism. LUJV is a novel, genetically distinct, highly pathogenic arenavirus.

引用

页数：8

共 79 条

[1] Arenavirus phylogeny:: A new insight [J].