Isolation and characterization of polymorphic microsatellite loci from a dinucleotide-enriched genomic library of obscure puffer (Takifugu obscurus) and cross-species amplification

被引：16
|
作者：
Ma, Hongyu [1 ,2 ]
Chen, Songlin [1 ]
Liao, Xiaolin [1 ]
Xu, Tianjun [1 ]
Ge, Jiachun [3 ]
机构：
[1] Chinese Acad Fishery Sci, Key Lab Sustainable Utilizat Marine Fisheries Res, Minist Agr, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
[2] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China
[3] Freshwater Fisheries Res Inst, Nanjing 210017, Peoples R China
来源：
CONSERVATION GENETICS | 2009年 / 10卷 / 04期
关键词：
Obscure puffer; Takifugu obscurus; Microsatellite; Enriched genomic library;
D O I：
10.1007/s10592-008-9540-2
中图分类号：
X176 [生物多样性保护];
学科分类号：
090705 ;
摘要：
Obscure puffer (Takifugu obscurus) is an anadromous fish species in China. Here, we reported 10 polymorphic microsatellite loci isolated from a dinucleotide-enriched genomic library of T. obscurus. The number of alleles, observed and expected heterozygosity per locus in 30 individuals ranged from four to 10, from 0.57 to 0.86 and from 0.68 to 0.90, respectively. Three loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction and no significant linkage disequilibrium between pairs of loci was found. Cross-species amplification of these microsatellite loci in additional three fish species was performed. These polymorphic microsatellite loci would be useful for investigating genetic population structure and construction of genetic linkage map in T. obscurus.
引用
收藏
页码：955 / 957
页数：3
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