Fluorogenic probes for live-cell imaging of the cytoskeleton

被引：0

作者：

Lukinavicius, Grazvydas ^{[1
]}

Reymond, Luc ^{[1
,2
]}

D'Este, Elisa ^{[3
]}

Masharina, Anastasiya ^{[1
]}

Gutfert, Fabian ^{[3
]}

Ta, Haisen ^{[3
]}

Guether, Angelika ^{[4
]}

Fournier, Mathias ^{[5
]}

Rizzo, Stefano ^{[6
]}

Waldmann, Herbert

Blaukopf, Claudia

Sommer, Christoph ^{[7
]}

Gerlich, Daniel W. ^{[7
]}

Arndt, Hans-Dieter ^{[4
]}

Hell, Stefan W. ^{[3
]}

Johnsson, Kai ^{[1
,2
]}

机构：

[1] Ecole Polytech Fed Lausanne, Inst Chem Sci & Engn, Lausanne, Switzerland

[2] Natl Ctr Competence Res Chem Biol, Lausanne, Switzerland

[3] Max Planck Inst Biophys Chem, Dept NanoBiophoton, D-37077 Gottingen, Germany

[4] Univ Jena, Inst Organ Chem & Macromol Chem, Jena, Germany

[5] Ecole Polytech Fed Lausanne, Lausanne, Switzerland

[6] Max Planck Inst Mol Physiol, D-44139 Dortmund, Germany

[7] Austrian Acad Sci, Inst Mol Biotechnol, A-1010 Vienna, Austria

来源：

NATURE METHODS | 2014年 / 11卷 / 07期

基金：

瑞士国家科学基金会; 欧洲研究理事会;

关键词：

FLUORESCENCE MICROSCOPY; ACTIN POLYMERIZATION; STIMULATED-EMISSION; RESOLUTION LIMIT; STED NANOSCOPY; F-ACTIN; BINDING; VISUALIZATION; MICROTUBULES; PHALLOTOXIN;

D O I：

10.1038/NMETH.2972

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We introduce far-red, fluorogenic probes that combine minimal cytotoxicity with excellent brightness and photostability for fluorescence imaging of actin and tubulin in living cells. Applied in stimulated emission depletion (STED) microscopy, they reveal the ninefold symmetry of the centrosome and the spatial organization of actin in the axon of cultured rat neurons with a resolution unprecedented for imaging cytoskeletal structures in living cells.

引用

页码：731 / U168

页数：7

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