An assessment of correlation between serum asymmetric dimethylarginine and glycated haemoglobin in patients with type 2 diabetes mellitus

Studies that investigated an association between asymmetric dimethylarginine (ADMA) and glycated haemoglobin (HbA(1c)) in type 2 diabetes mellitus (T2DM) have given discordant results. The aim of this study was to determine and compare serum ADMA concentration in patients with T2DM and healthy controls, and to assess correlation between ADMA and HbA(1c) in patients with T2DM. Serum ADMA concentration was determined by ELISA method with the use of ADMA (center dot) - ELISA kit (DLD Diagnostics, Hamburg, Germany) and HbA(1c) levels were determined by an immunoturbidimetric method in 60 patients with T2DM and 60 healthy individuals matched for age and sex. Results have shown that mean serum ADMA concentration was significantly higher in T2DM patients (1.54 +/- 0.06 mu mol/L) compared to mean serum ADMA concentration (0.62 +/- 0.02 mu mol/L;p<0.000l) in healthy subjects. A significant, positive, correlation between serum ADMA concentration and HbA(1c) levels was observed (r=0.494;p<0.01) in T2DM patients. Our results suggest that there is an association between endothelial dysfunction and glycaemic control in type 2 diabetes mellitus. Possible explanation for obtained results may be oxidative stress that is increased in conditions of hyperglycaemia and it also promotes endothelial dysfunction. Larger, longitudinal studies are required that will evaluate relation between metabolic abnormalities and increased ADMA levels in patients with type a diabetes mellitus. (C) 2014 Association of Basic Medical Sciences of FB&H. All rights reserved