Novel coding, translation, and gene expression of a replicating covalently closed circular RNA of 220 nt

被引：154
作者：
AbouHaidar, Mounir Georges [1 ]
Venkataraman, Srividhya [1 ]
Golshani, Ashkan [2 ]
Liu, Bolin [1 ]
Ahmad, Tauqeer [1 ]
机构：
[1] Univ Toronto, Dept Cell & Syst Biol, Toronto, ON M5S 3B2, Canada
[2] Carleton Univ, Dept Biol, Ottawa, ON K1S 5B6, Canada
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2014年 / 111卷 / 40期
关键词：
hammerhead ribozyme; sobemovirus; circular RNA translation; leaky termination codons; YELLOW-MOTTLE VIRUS; SATELLITE RNA; MESSENGER-RNA; OVERLAPPING GENES; VIROIDS; PROTEIN; TRANSCRIPTION; EUKARYOTES; SEQUENCES; PLANTS;
D O I：
10.1073/pnas.1402814111
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The highly structured (64% GC) covalently closed circular (CCC) RNA (220 nt) of the virusoid associated with rice yellow mottle virus codes for a 16-kDa highly basic protein using novel modalities for coding, translation, and gene expression. This CCC RNA is the smallest among all known viroids and virusoids and the only one that codes proteins. Its sequence possesses an internal ribosome entry site and is directly translated through two (or three) completely overlapping ORFs (shifting to a new reading frame at the end of each round). The initiation and termination codons overlap UGAUGA (underline highlights the initiation codon AUG within the combined initiation-termination sequence). Termination codons can be ignored to obtain larger read-through proteins. This circular RNA with no noncoding sequences is a unique natural supercompact "nanogenome."
引用
收藏
页码：14542 / 14547
页数：6
相关论文
共 27 条
  • [1] COMPARISON OF THE NUCLEOTIDE-SEQUENCES OF VIROID-LIKE SATELLITE RNA OF THE CANADIAN AND AUSTRALASIAN STRAINS OF LUCERNE TRANSIENT STREAK VIRUS
    ABOUHAIDAR, MG
    PALIWAL, YC
    [J]. JOURNAL OF GENERAL VIROLOGY, 1988, 69 : 2369 - 2373
  • [2] OVERLAPPING GENES IN BACTERIOPHAGE-PSIX174
    BARRELL, BG
    AIR, GM
    HUTCHISON, CA
    [J]. NATURE, 1976, 264 (5581) : 34 - 41
  • [3] BRANCH AD, 1984, SCIENCE, V223, P450, DOI 10.1126/science.6197756
  • [4] REPLICATION OF A PLANT-VIRUS SATELLITE RNA - EVIDENCE FAVORS TRANSCRIPTION OF CIRCULAR TEMPLATES OF BOTH POLARITIES
    BRUENING, G
    PASSMORE, BK
    VANTOL, H
    BUZAYAN, JM
    FELDSTEIN, PA
    [J]. MOLECULAR PLANT-MICROBE INTERACTIONS, 1991, 4 (03) : 219 - 225
  • [5] Self-cleaving circular RNA associated with rice yellow mottle virus is the smallest viroid-like RNA
    Collins, RF
    Gellatly, DL
    Sehgal, OP
    Abouhaidar, MG
    [J]. VIROLOGY, 1998, 241 (02) : 269 - 275
  • [6] VIROIDS
    DIENER, TO
    [J]. ADVANCES IN VIRUS RESEARCH, 1983, 28 : 241 - 283
  • [7] Viroids and viroid-host interactions
    Flores, R
    Hernández, C
    Martínez de Alba, AE
    Daròs, JA
    Di Serio, F
    [J]. ANNUAL REVIEW OF PHYTOPATHOLOGY, 2005, 43 : 117 - +
  • [8] SELF-CLEAVAGE OF PLUS AND MINUS RNAS OF A VIRUSOID AND A STRUCTURAL MODEL FOR THE ACTIVE-SITES
    FORSTER, AC
    SYMONS, RH
    [J]. CELL, 1987, 49 (02) : 211 - 220
  • [9] Structural and sequence integrity are essential for the replication of the viroid-like satellite RNA of lucerne transient streak virus
    Gellatly, Duncan
    Mirhadi, Kayvan
    Venkataraman, Srividhya
    AbouHaidar, Mounir G.
    [J]. JOURNAL OF GENERAL VIROLOGY, 2011, 92 : 1475 - 1481
  • [10] Gibbs AJ., 1995, Molecular basis of virus evolution
123