Novel coding, translation, and gene expression of a replicating covalently closed circular RNA of 220 nt

被引：158

作者：

AbouHaidar, Mounir Georges ^{[1
]}

Venkataraman, Srividhya ^{[1
]}

Golshani, Ashkan ^{[2
]}

Liu, Bolin ^{[1
]}

Ahmad, Tauqeer ^{[1
]}

机构：

[1] Univ Toronto, Dept Cell & Syst Biol, Toronto, ON M5S 3B2, Canada

[2] Carleton Univ, Dept Biol, Ottawa, ON K1S 5B6, Canada

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2014年 / 111卷 / 40期

关键词：

hammerhead ribozyme; sobemovirus; circular RNA translation; leaky termination codons; YELLOW-MOTTLE VIRUS; SATELLITE RNA; MESSENGER-RNA; OVERLAPPING GENES; VIROIDS; PROTEIN; TRANSCRIPTION; EUKARYOTES; SEQUENCES; PLANTS;

D O I：

10.1073/pnas.1402814111

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The highly structured (64% GC) covalently closed circular (CCC) RNA (220 nt) of the virusoid associated with rice yellow mottle virus codes for a 16-kDa highly basic protein using novel modalities for coding, translation, and gene expression. This CCC RNA is the smallest among all known viroids and virusoids and the only one that codes proteins. Its sequence possesses an internal ribosome entry site and is directly translated through two (or three) completely overlapping ORFs (shifting to a new reading frame at the end of each round). The initiation and termination codons overlap UGAUGA (underline highlights the initiation codon AUG within the combined initiation-termination sequence). Termination codons can be ignored to obtain larger read-through proteins. This circular RNA with no noncoding sequences is a unique natural supercompact "nanogenome."

引用

页码：14542 / 14547

页数：6

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