Polymerase chain reaction for sexing in giant anteater's (Myrmecophaga tridactyla)

被引：2

作者：

Bento, H. J. ^{[1
]}

Rosa, J. M. A. ^{[1
]}

Morgado, T. O. ^{[2
]}

Granjeiro, B. ^{[2
]}

Bianchini, M. A. ^{[2
]}

Iglesias, G. A. ^{[1
]}

Dutra, V ^{[1
]}

Nakazato, L. ^{[1
]}

Paz, R. C. R. ^{[1
]}

机构：

[1] Univ Fed Mato Grosso, FAVET, Programa Posgrad, Cuiaba, MT, Brazil

[2] Univ Fed Mato Grosso, FAVET, Fac Med Vet, Cuiaba, MT, Brazil

来源：

ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA | 2019年 / 71卷 / 02期

关键词：

pilosa; reproduction; conservation; sexing determination; DNA; EMAS NATIONAL-PARK; DNA;

D O I：

10.1590/1678-4162-9923

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

There is no sexual dimorphism in the giant anteater (Myrmecophaga tridactyla), so the distinction between males and females become necessary, especially in animals with reproductive purpose. The Polymerase Chain Reaction (PCR), among the various techniques used for characterization, is considered a modern and effective means of sex determination and used in mammals to identify the Y chromosome (SRY) specifies genetic sequence. The objective of this work is to standardize a protocol for sex determination of giant anteater by PCR technique, using genetic material extracted from the capillary bulb of these animals. With this protocol was possible the sex determination of seven tested animals, being compatible with the sex of each individual. In conclusion, this protocol showed total effectiveness, being possible to determine the giant anteater sex using genetic material extracted from the capillary bulb.

引用

页码：538 / 544

页数：7

共 26 条

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