Expression of mRNA and protein of IL-18 and its receptor in human follicular granulosa cells

被引：14
|
作者：
Salmassi, A. [1 ,2 ]
Fattahi, A. [4 ]
Nouri, M. [1 ]
Hedderich, J. [3 ]
Schmutzler, A. G. [2 ]
机构：
[1] Tabriz Univ Med Sci, Fac Adv Med Sci, Dept Reprod Biol, Tabriz, Iran
[2] Univ Kiel, Dept Obstet & Gynaecol, Ctr Reprod Med, Womens Hosp,Univ Hosp Schleswig Holstein, Campus Kiel,Arnold Heller Str 3,House 24, D-24105 Kiel, Germany
[3] Univ Kiel, Inst Med Informat & Stat, Kiel, Germany
[4] Tabriz Univ Med Sci, Womens Reprod Hlth Res Ctr, Tabriz, Iran
来源：
JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION | 2017年 / 40卷 / 04期
关键词：
Granulosa cells; IL-18; receptor; IVFICSI; COLONY-STIMULATING FACTOR; IN-VITRO FERTILIZATION; GAMMA-INDUCING FACTOR; POLYCYSTIC-OVARY-SYNDROME; INTERLEUKIN-18; LEVELS; INSULIN-RESISTANCE; INTERFERON-GAMMA; BINDING-PROTEIN; TH2; RESPONSES; CYTOKINE;
D O I：
10.1007/s40618-016-0590-x
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Purpose There is no information available about the IL-18 receptor in ovarian follicles, so the present study attempts to demonstrate the expression of IL-18 and its receptor in human granulosa cells (GCs). Methods To evaluate the concentration of IL-18 in serum and follicular fluid (FF), we collected serum and FF from 102 women undergoing oocyte retrieval. Also, to detect expression of IL-18 and its receptor by luteinized GCs, these cells were pooled six times from a total of twenty individual patients with 5-16 follicles each. The IL-18 concentration was determined by ELISA and the expression of IL-18 and its receptor by immunocytochemistry and reverse transcription polymerase chain reaction. Results Our results showed that the median IL-18 concentration in serum, 159.27 pg/ml (IQR 121.41-210.1), was significantly higher than in FF, 142.1 pg/ml (IQR 95.7176.5), p < 0.001. Moreover, we found that IL-18 and its receptor are expressed by GCs. Conclusion The presence of IL-18 in FF and the expression of IL-18 and its receptor by GCs suggest an important role for this cytokine in ovarian function.
引用
收藏
页码：447 / 454
页数：8
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