Expression of mRNA and protein of IL-18 and its receptor in human follicular granulosa cells

被引：14
|
作者：
Salmassi, A. [1 ,2 ]
Fattahi, A. [4 ]
Nouri, M. [1 ]
Hedderich, J. [3 ]
Schmutzler, A. G. [2 ]
机构：
[1] Tabriz Univ Med Sci, Fac Adv Med Sci, Dept Reprod Biol, Tabriz, Iran
[2] Univ Kiel, Dept Obstet & Gynaecol, Ctr Reprod Med, Womens Hosp,Univ Hosp Schleswig Holstein, Campus Kiel,Arnold Heller Str 3,House 24, D-24105 Kiel, Germany
[3] Univ Kiel, Inst Med Informat & Stat, Kiel, Germany
[4] Tabriz Univ Med Sci, Womens Reprod Hlth Res Ctr, Tabriz, Iran
来源：
JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION | 2017年 / 40卷 / 04期
关键词：
Granulosa cells; IL-18; receptor; IVFICSI; COLONY-STIMULATING FACTOR; IN-VITRO FERTILIZATION; GAMMA-INDUCING FACTOR; POLYCYSTIC-OVARY-SYNDROME; INTERLEUKIN-18; LEVELS; INSULIN-RESISTANCE; INTERFERON-GAMMA; BINDING-PROTEIN; TH2; RESPONSES; CYTOKINE;
D O I：
10.1007/s40618-016-0590-x
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Purpose There is no information available about the IL-18 receptor in ovarian follicles, so the present study attempts to demonstrate the expression of IL-18 and its receptor in human granulosa cells (GCs). Methods To evaluate the concentration of IL-18 in serum and follicular fluid (FF), we collected serum and FF from 102 women undergoing oocyte retrieval. Also, to detect expression of IL-18 and its receptor by luteinized GCs, these cells were pooled six times from a total of twenty individual patients with 5-16 follicles each. The IL-18 concentration was determined by ELISA and the expression of IL-18 and its receptor by immunocytochemistry and reverse transcription polymerase chain reaction. Results Our results showed that the median IL-18 concentration in serum, 159.27 pg/ml (IQR 121.41-210.1), was significantly higher than in FF, 142.1 pg/ml (IQR 95.7176.5), p < 0.001. Moreover, we found that IL-18 and its receptor are expressed by GCs. Conclusion The presence of IL-18 in FF and the expression of IL-18 and its receptor by GCs suggest an important role for this cytokine in ovarian function.
引用
收藏
页码：447 / 454
页数：8
相关论文
共 50 条
  • [11] IL-18 and IL-18 binding protein concentration in ovarian follicular fluid of women with "tubal factor" subjected to in vitro fertilization
    Radwan, Pawel
    Radwan, Michal
    Polac, Ireneusz
    Lewkowicz, Przemyslaw
    Wilczynski, Jacek Radoslaw
    POSTEPY HIGIENY I MEDYCYNY DOSWIADCZALNEJ, 2013, 67 : 463 - 470
  • [12] Enhanced Expression of IL-18 and IL-18BP in Plasma of Patients with Eczema: Altered Expression of IL-18BP and IL-18 Receptor on Mast Cells
    Hu, Yalin
    Wang, Junling
    Zhang, Huiyun
    Xie, Hua
    Song, Weiwei
    Jiang, Qijun
    Zhao, Nan
    He, Shaoheng
    MEDIATORS OF INFLAMMATION, 2017, 2017
  • [13] Structural basis of human IL-18 sequestration by the decoy receptor IL-18 binding protein in inflammation and tumor immunity
    Detry, Sam
    Andries, Julie
    Bloch, Yehudi
    Gabay, Cem
    Clancy, Danielle M.
    Savvides, Savvas N.
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2022, 298 (05)
  • [14] IL-18 and IL-18 binding protein concentration in ovarian follicular fluid of women with unexplained infertility to PCOS during in vitro fertilization
    Zhang, Hongyuan
    Wang, Xinmei
    Xu, Juan
    Zhu, Yingjun
    Chen, Xu
    Hu, Yuanjing
    JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 2020, 138
  • [15] Increased expression of PGRN protein in follicular fluid and mRNA in granulosa cells in overweight patients with polycystic ovary syndrome
    Zhou, Danni
    Li, Saijiao
    Li, Wei
    Yin, Tailang
    Xu, Wangming
    Zhang, Jun
    Yang, Jing
    EUROPEAN JOURNAL OF OBSTETRICS & GYNECOLOGY AND REPRODUCTIVE BIOLOGY, 2017, 218 : 106 - 112
  • [16] Differential synthesis and release of IL-18 and IL-18 Binding Protein from human platelets and their implications for HIV infection
    Allam, Ossama
    Samarani, Suzanne
    Jenabian, Mohammad-Ali
    Routy, Jean-Pierre
    Tremblay, Cecile
    Amre, Devendra
    Ahmad, Ali
    CYTOKINE, 2017, 90 : 144 - 154
  • [17] Interleukin-18, IL-18 binding protein and IL-18 receptor expression in asthma: a hypothesis showing IL-18 promotes epithelial cell differentiation
    Kaur, Davinder
    Chachi, Latifa
    Gomez, Edith
    Sylvius, Nicolas
    Brightling, Christopher E.
    CLINICAL & TRANSLATIONAL IMMUNOLOGY, 2021, 10 (06)
  • [18] Increased expression of IL-18 in the serum and islets of type 1 diabetics
    Harms, Robert Z.
    Yarde, Danielle N.
    Guinn, Zachary
    Lorenzo-Arteaga, Kristina M.
    Corley, Kevin P.
    Cabrera, Monina S.
    Sarvetnick, Nora E.
    MOLECULAR IMMUNOLOGY, 2015, 64 (02) : 306 - 312
  • [19] Allergens elicit upregulated IL-18 and IL-18Rα expression in blood monocytes of patients with allergic rhinitis
    Wang, Junling
    Gu, Fangqiu
    Li, Zhi
    Zhan, Mengmeng
    Wang, Ling
    Zhang, Huiyun
    Chen, Dong
    Xie, Hua
    Chai, Ruonan
    Zhao, Nan
    Yang, Ruiming
    He, Shaoheng
    SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 2022, 96 (06)
  • [20] The IL-18 Antagonist IL-18-Binding Protein Is Produced in the Human Ovarian Cancer Microenvironment
    Carbotti, Grazia
    Barisione, Gaia
    Orengo, Anna Maria
    Brizzolara, Antonella
    Airoldi, Irma
    Bagnoli, Marina
    Pinciroli, Patrizia
    Mezzanzanica, Delia
    Centurioni, Maria Grazia
    Fabbi, Marina
    Ferrini, Silvano
    CLINICAL CANCER RESEARCH, 2013, 19 (17) : 4611 - 4620
12345