Screening of estrogenic endocrine-disrupting chemicals in meat products based on the detection of vitellogenin by enzyme-linked immunosorbent assay

被引：8

作者：

Li, Xiaoqi ^{[1
]}

Liu, Xiaofan ^{[1
]}

Jia, Zhenzhen ^{[1
]}

Wang, Tian ^{[1
]}

Zhang, Hongyan ^{[1
]}

机构：

[1] Shandong Normal Univ, Key Lab Food Nutr & Safety, Coll Life Sci, Shandong Prov Key Lab Anim Resistance Biol,Inst B, Jinan 250014, Peoples R China

来源：

CHEMOSPHERE | 2021年 / 263卷

基金：

中国国家自然科学基金;

关键词：

Estrogenic endocrine-disrupting chemicals; Vitellogenin; Enzyme-linked immunosorbent assay; Meat products; Dimethyl terephthalate;

D O I：

10.1016/j.chemosphere.2020.128251

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Vitellogenin (VTG) is typically produced by females but when present in males can indicate contamination with estrogenic endocrine-disrupting chemicals (EDCs). Here we used primary hepatocytes isolated from male crucian carp uncontaminated with estrogenic EDCs as a culture model. Nine EDCs were used for validation, and an enzyme-linked immunosorbent assay (ELISA) was used to quantify VTG production by primary hepatocytes. The lower limit of detection of 17 beta-E2, DES, and HES using the method was 10(-12) M. Fresh pork was roasted, and non-hydrolytic acetonitrile-vortexing was used to extract potential estrogenic EDCs. The extracted substances were separated by thin-layer chromatography (TLC), and dimethyl terephthalate present in roast pork was found to induce high VTG concentrations. Therefore, the detection of VTG by ELISA provides a sensitive and reliable method for detecting known and unknown estrogenic EDCs in food products. (C) 2020 Elsevier Ltd. All rights reserved.

引用

页数：7

共 21 条

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