(-)-Epicatechin-3-gallate (a polyphenol from green tea) potentiates doxorubicin-induced apoptosis in H9C2 cardiomyocytes

被引：10
作者：
Cai, Yi [1 ]
Hong, Hui-Qi [2 ]
Zhao, Li [1 ]
Cai, Yue-Peng [3 ]
Qin, Yuan [1 ]
机构：
[1] Guangzhou Med Univ, Guangzhou Res Inst Snake Venom, Guangzhou 510182, Guangdong, Peoples R China
[2] Sun Yat Sen Univ, Sch Pharmaceut Sci, Dept Pharmacol & Toxicol, Guangzhou 510006, Guangdong, Peoples R China
[3] S China Normal Univ, Sch Chem & Environm, Guangzhou Key Lab Mat Energy Convers & Storage, Guangzhou 510006, Guangdong, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2015年 / 37卷 / 10期
基金：
中国国家自然科学基金;
关键词：
AMP-activated protein kinase; Apoptosis; Cardiomyocytes; Doxorubicin; Epicatechin-3-gallate; Green tea polyphenol; H9C2; cardiomyocytes; CANCER;
D O I：
10.1007/s10529-015-1879-0
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The effect of epicatechin-3-gallate (ECG), a polyphenol that is present in green tea, on doxorubicin (DOX) cytotoxicity in H9C2 cardiomyocytes and its underlying mechanisms were investigated. Pretreatment with ECG (20 and 30 mu M) significantly increased DOX-induced apoptosis to 16-18 % in H9C2 cardiomyocytes. The Bax/Bcl-2 ratio increased significantly after 1 h pretreatment with ECG. ECG also enhanced the phosphorylation of AMP-activated protein kinase (AMPK) which was induced by DOX in a dose-dependent manner. Pretreatment with ECG sensitized H9C2 cells to DOX-mediated apoptosis through modulation of proteins involved in apoptosis and AMPK.
引用
收藏
页码：1937 / 1943
页数：7
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