A Surface Plasmon Resonance Spectroscopy Method for Characterizing Small-Molecule Binding to Nerve Growth Factor

被引：4

作者：

Kennedy, Allison E. ^{[1
]}

Sheffield, Kristen S. ^{[2
]}

Eibl, Joseph K. ^{[3
]}

Murphy, Michael B. ^{[4
]}

Vohra, Rahul ^{[5
]}

Scott, John A. ^{[1
]}

Ross, Gregory M. ^{[2
,3
]}

机构：

[1] Laurentian Univ, Biomol Sci Program, Sudbury, ON P3E 2C6, Canada

[2] Laurentian Univ, Dept Biol, Sudbury, ON P3E 2C6, Canada

[3] Northern Ontario Sch Med, Sudbury, ON P3E 2C6, Canada

[4] GE Healthcare Life Sci, Piscataway, NJ USA

[5] Sussex Res, Ottawa, ON, Canada

来源：

JOURNAL OF BIOMOLECULAR SCREENING | 2016年 / 21卷 / 01期

关键词：

neurotrophin; NGF; pain therapeutics; SPR; biosensor technology; PAIN; ANTAGONIST; NGF; TRKA;

D O I：

10.1177/1087057115607814

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Small-molecule inhibitors have been previously investigated to identify possible therapeutics for the treatment of chronic pain. In the present study, known nerve growth factor (NGF) inhibitors identified by I-125-NGF binding were characterized using affinity and binding evaluations by surface plasmon resonance (SPR) spectroscopy. A novel strategy for characterizing NGF inhibitors was used to determine the binding affinity (K-D) and saturation ability of each compound with immobilized NGF. Seventy-four percent of compounds screened demonstrated a positive binding event to NGF. A K-D less than 10M and a percent saturation greater than 50% were used as thresholds to identify inhibitors that would warrant further investigation. This study details for the first time a methodology that can be used to directly characterize the binding event between small-molecule inhibitors and NGF.

引用

页码：96 / 100

页数：5