Chikungunya virus superinfection exclusion is mediated by a block in viral replication and does not rely on non-structural protein 2

被引:11
作者
Boussier, Jeremy [1 ,2 ,3 ]
Levi, Laura [4 ]
Weger-Lucarelli, James [4 ,5 ]
Poirier, Enzo Z. [4 ,6 ]
Vignuzzi, Marco [4 ]
Albert, Matthew L. [1 ,7 ]
机构
[1] Inst Pasteur, Immubiol Dendrit Cells Unit, Paris, France
[2] Inst Pasteur, Inserm U1223, Paris, France
[3] Univ Paris Diderot, Ecole Doctorale Frontieres Vivant, Paris, France
[4] Inst Pasteur, Viral Populat & Pathogenesis Unit, Paris, France
[5] Virginia Tech, Dept Biomed Sci & Pathobiol, VA MD Reg Coll Vet Med, Blacksburg, VA USA
[6] Francis Crick Inst, Immunobiol Lab, London, England
[7] Insitro, San Francisco, CA 94080 USA
关键词
CELL-SURFACE CD4; MESSENGER-RNA; CROSS-PROTECTION; INFECTION; INTERFERENCE; MECHANISMS; EXPRESSION; STRAINS; KINASE; NEF;
D O I
10.1371/journal.pone.0241592
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Superinfection exclusion (SIE) is a process by which a virally infected cell is protected from subsequent infection by the same or a closely related virus. By preventing cell coinfection, SIE favors preservation of genome integrity of a viral strain and limits its recombination potential with other viral genomes, thereby impacting viral evolution. Although described in virtually all viral families, the precise step(s) impacted by SIE during the viral life cycle have not been systematically explored. Here, we describe for the first time SIE triggered by chikungunya virus (CHIKV), an alphavirus of public health importance. Using single-cell technologies, we demonstrate that CHIKV excludes subsequent infection with: CHIKV; Sindbis virus, a related alphavirus; and influenza A, an unrelated RNA virus. We further demonstrate that SIE does not depend on the action of type I interferon, nor does it rely on host cell transcription. Moreover, exclusion is not mediated by the action of a single CHIKV protein; in particular, we observed no role for non-structural protein 2 (nsP2), making CHIKV unique among characterized alphaviruses. By stepping through the viral life cycle, we show that CHIKV exclusion occurs at the level of replication, but does not directly influence virus binding, nor viral structural protein translation. In sum, we characterized co-infection during CHIKV replication, which likely influences the rate of viral diversification and evolution.
引用
收藏
页数:22
相关论文
共 50 条
[21]   Insights into the role of the non-structural protein 2 (NS2) in Bluetongue virus morphogenesis [J].
Butan, Carmen ;
Tucker, Paul .
VIRUS RESEARCH, 2010, 151 (02) :109-117
[22]   Non-Structural Protein V of Canine Distemper Virus Induces Autophagy via PI3K/AKT/mTOR Pathway to Facilitate Viral Replication [J].
Tian, Xin ;
Zhang, Rui ;
Yi, Shuang ;
Chen, Yu ;
Jiang, Ying ;
Zhang, Xianwen ;
Zhang, Zhidong ;
Li, Yanmin .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2025, 26 (01)
[23]   Rad51 Interacts with Non-structural 3 Protein of Hepatitis C Virus and Regulates Viral Production [J].
Son, Kidong ;
Nguyen, Tram T. T. ;
Choi, Jae-Woong ;
Pham, Long V. ;
Luong, Trang T. D. ;
Lim, Yun-Sook ;
Hwang, Soon B. .
FRONTIERS IN MICROBIOLOGY, 2017, 8
[24]   RETRACTED: Host protein CD63 promotes viral RNA replication by interacting with human astrovirus non-structural protein nsP1a/4 (Retracted Article) [J].
Zhao, Wei ;
Tao, Xiao Li ;
Liu, Nian ;
Lu, Heng Zhang ;
Zheng, Chun Hong ;
Li, Xiang Yu ;
Yu, Man ;
Li, Yong Gang .
JOURNAL OF GENERAL VIROLOGY, 2019, 100 (04) :616-628
[25]   BST-2/tetherin-mediated restriction of chikungunya (CHIKV) VLP budding is counteracted by CHIKV non-structural protein 1 (nsP1) [J].
Jones, Philip H. ;
Maric, Martina ;
Madison, Marisa N. ;
Maury, Wendy ;
Roller, Richard J. ;
Okeoma, Chioma M. .
VIROLOGY, 2013, 438 (01) :37-49
[26]   Inhibitors of Venezuelan Equine Encephalitis Virus Identified Based on Host Interaction Partners of Viral Non-Structural Protein 3 [J].
Bakovic, Allison ;
Bhalla, Nishank ;
Alem, Farhang ;
Campbell, Catherine ;
Zhou, Weidong ;
Narayanan, Aarthi .
VIRUSES-BASEL, 2021, 13 (08)
[27]   Hepatitis C virus non-structural proteins modulate cellular kinases for increased cytoplasmic abundance of host factor HuR and facilitate viral replication [J].
Raheja, Harsha ;
George, Biju ;
Tripathi, Sachin Kumar ;
Saha, Sandhini ;
Maiti, Tushar Kanti ;
Das, Saumitra .
PLOS PATHOGENS, 2023, 19 (08)
[28]   Non-Structural Protein-W61 as a Novel Target in Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV): An In-Vitro and In-Silico Study on Protein-Protein Interactions with Nucleoprotein and Viral Replication [J].
Park, Ji-Young ;
Sivasankar, Chandran ;
Kirthika, Perumalraja ;
Prabhu, Dhamodharan ;
Lee, John Hwa .
VIRUSES-BASEL, 2023, 15 (09)
[29]   Viroporin Activity of the Foot-and-Mouth Disease Virus Non-Structural 2B Protein [J].
Ao, Da ;
Guo, Hui-Chen ;
Sun, Shi-Qi ;
Sun, De-Hui ;
Fung, To Sing ;
Wei, Yan-Quan ;
Han, Shi-Chong ;
Yao, Xue-Ping ;
Cao, Sui-Zhong ;
Liu, Ding Xiang ;
Liu, Xiang-Tao .
PLOS ONE, 2015, 10 (05)
[30]   Cellular microRNA miR-181b Inhibits Replication of Mink Enteritis Virus by Repression of Non-Structural Protein 1 Translation [J].
Sun, Jia-zeng ;
Wang, Jigui ;
Yuan, Daoli ;
Wang, Shuang ;
Li, Zhili ;
Yi, Bao ;
Mao, Yaping ;
Hou, Qiang ;
Liu, Weiquan .
PLOS ONE, 2013, 8 (12)