Manipulation of the physiology of clavulanic acid production in Streptomyces clavuligerus

被引:45
作者
Ives, PR [1 ]
Bushell, ME [1 ]
机构
[1] UNIV SURREY,MICROBIAL PHYSIOL & ECOL GRP,GUILDFORD GU2 5XH,SURREY,ENGLAND
来源
MICROBIOLOGY-UK | 1997年 / 143卷
关键词
secondary metabolism; clavulanic acid; nutrient feeds; cluster analysis;
D O I
10.1099/00221287-143-11-3573
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
This paper reports a novel use of cluster analysis for the identification of intermediary metabolites that are produced at rates closely correlated with theme of antibiotic biosynthesis. This information was used to devise culture feeds resulting in enhanced production of clavulanic acid, an antibiotic of current worldwide commercial interest. The feeding strategies apparently alleviated a rate-limiting supply of the C-3 precursor of clavulanic acid. C-3 limitation may be a consequence of unusual nitrogen and carbon metabolism in Streptomyces clavuligerus. This approach has potential as a generic method for influencing biosynthetic pathway fluxes using feeds without knowledge of the biosynthetic pathway.
引用
收藏
页码:3573 / 3579
页数:7
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