Adenosine triphosphate, polymyxin B and B16 cell-derived immunization induce anticancer response

被引：2

作者：

Barrera-Avalos, Carlos ^{[1
,2
]}

Mena, Javier ^{[1
]}

Lopez, Ximena ^{[1
]}

Cappelli, Claudio ^{[1
,6
]}

Neira, Tanya ^{[1
]}

Imarai, Monica ^{[1
,2
]}

Fernandez, Ricardo ^{[3
]}

Robles-Planells, Claudia ^{[1
,2
]}

Rojo, Leonel E. ^{[1
,2
]}

Milla, Luis A. ^{[4
]}

Leiva-Salcedo, Elias ^{[1
]}

Escobar, Alejandro ^{[5
]}

Acuna-Castillo, Claudio ^{[1
,2
]}

机构：

[1] Univ Santiago Chile, USACH, Fac Quim & Biol, Dept Biol, Alameda 3363, Santiago, Chile

[2] Univ Santiago Chile, USACH, Ctr Biotecnol Acuicola, Alameda 3363, Santiago, Chile

[3] Univ Lagos, Dept Salud, Osorno, Chile

[4] Univ Santiago Chile, Fac Ciencias Med, Escuela Med, Ctr Invest Biomed & Aplica, Santiago, Chile

[5] Univ Chile, Fac Odontol, Inst Invest Ciencias Odontol, Santiago, Chile

[6] Univ Austral Chile, Inst Bioquim & Microbiol, Lab Patol Mol, Valdivia, Chile

来源：

IMMUNOTHERAPY | 2021年 / 13卷 / 04期

关键词：

anticancer; ATP; cost-effective; DAMPs; immunotherapy; melanoma; polymyxin B; P2X(7) RECEPTOR; CALRETICULIN EXPOSURE; DENDRITIC CELLS; TUMOR-GROWTH; IMMUNOGENICITY; AUTOPHAGY; DEATH;

D O I：

10.2217/imt-2020-0209

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Aim: Whole dead tumor cells can be used as antigen source and the induction of protective immune response could be enhanced by damage-associated molecular patterns. Materials & methods: We generated whole dead tumor cells called B16-immunogenic cell bodies (ICBs) from B16 melanoma cells by nutrient starvation and evaluated the in vivo antitumor effect of B16-ICBs plus ATP and polymyxin B (PMB). Results: The subcutaneous immunization with B16-ICBs + PMB + ATP a 50% of tumor-free animals and induced a significant delay in tumor growth in a prophylactic approach. These results correlated with maturation of bone marrow-derived dendritic cells and activation of T CD8(+) lymphocytes in vitro. Conclusion: Altogether, ICB + ATP + PMB is efficient in inducing the antitumor efficacy of the whole dead tumor cells vaccine.

引用

页码：309 / 326

页数：18

共 64 条

[1] Vaccination with Necroptotic Cancer Cells Induces Efficient Anti-tumor Immunity [J].