Therapeutic angiogenesis by autologous adipose-derived regenerative cells: comparison with bone marrow mononuclear cells

被引：49

作者：

Hao, Changning ^{[1
]}

Shintani, Satoshi ^{[1
]}

Shimizu, Yuuki ^{[1
]}

Kondo, Kazuhisa ^{[1
]}

Ishii, Masakazu ^{[1
]}

Wu, Hongxian ^{[1
]}

Murohara, Toyoaki ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Med, Dept Cardiol, Nagoya, Aichi 4668550, Japan

来源：

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 2014年 / 307卷 / 06期

关键词：

peripheral artery disease; adipose-derived regenerative cell; therapeutic angiogenesis; macrophage polarization; ENDOTHELIAL PROGENITOR CELLS; POSTNATAL NEOVASCULARIZATION; MACROPHAGE ACTIVATION; STEM-CELLS; TRANSPLANTATION; IL-10; IMPLANTATION; PLASTICITY; ISCHEMIA; TISSUE;

D O I：

10.1152/ajpheart.00310.2014

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Transplantation of adipose-derived regenerative cell (ADRC) enhances ischemia-induced angiogenesis, but the underlying mechanism remains unknown. Here, we compared the efficacy between ADRC and bone marrow mononuclear cell (BM-MNC) transplantation in rabbits model of hindlimb ischemia and examined the possible roles of alternative phenotypic macrophages polarization in ADRC-mediated angiogenesis using mice model of hindlimb ischemia. ADRCs and BM-MNCs were isolated from New Zealand White rabbits and C57BL/6J mice. In rabbit studies, our data showed that ADRCs could incorporate into the endothelial vasculature in vitro and in vivo. Both ADRC-conditioned media (CM) and BM-MNC-CM enhanced the migratory ability and interrupted the process of apoptosis in human umbilical vein endothelial cells. Four weeks after cell transplantation, augmentation of postnatal neovascularization was observed in the ischemic muscle injected with either ADRCs or BM-MNCs. In mice studies, we presented that ADRCs polarized into the IL-10-releasing M2 macrophages through PGE2-EP2/4 axis and suppressed the expressions of TNF-alpha and IL-6 in the ischemic muscle. Gene expressions of several angiogenic cytokines were amplified in the macrophages cultured in ADRC-CM rather than BM-MNC-CM. Blockade of IL-10 using neutralizing MAb attenuated the ADRC-mediated angiogenesis and caused muscle apoptosis in vivo. In conclusion, ADRC transplantation harvested similar effect of neovascularization augmentation compared with BM-MNC in experimental rabbit model of hindlimb ischemia; ADRC displayed a unique immunoregulatory manner of accelerating IL-10-releasing M2 macrophages polarization through the PGE2-EP2/4 axis.

引用

页码：H869 / H879

页数：11

共 36 条

[21] Bone marrow stromal cells attenuate sepsis via prostaglandin E2-dependent reprogramming of host macrophages to increase their interleukin-10 production [J].

Nemeth, Krisztian ;

Leelahavanichkul, Asada ;

Yuen, Peter S. T. ;

Mayer, Balazs ;

Parmelee, Alissa ;

Doi, Kent ;

Robey, Pamela G. ;

Leelahavanichkul, Kantima ;

Koller, Beverly H. ;

Brown, Jared M. ;

Hu, Xuzhen ;

Jelinek, Ivett ;

Star, Robert A. ;

Mezey, Eva .

NATURE MEDICINE, 2009, 15 (01) :42-49

[22] Plasticity of human adipose lineage cells toward endothelial cells -: Physiological and therapeutic perspectives [J].

Planat-Benard, V ;

Silvestre, JS ;

Cousin, B ;

André, M ;

Nibbelink, M ;

Tamarat, R ;

Clergue, M ;

Manneville, C ;

Saillan-Barreau, C ;

Duriez, M ;

Tedgui, A ;

Levy, B ;

Pènicaud, L ;

Casteilla, L .

CIRCULATION, 2004, 109 (05) :656-663

[23] Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells [J].

Rehman, J ;

Traktuev, D ;

Li, JL ;

Merfeld-Clauss, S ;

Temm-Grove, CJ ;

Bovenkerk, JE ;

Pell, CL ;

Johnstone, BH ;

Considine, RV ;

March, KL .

CIRCULATION, 2004, 109 (10) :1292-1298

[24] Macrophage diversity in renal injury and repair [J].

Ricardo, Sharon D. ;

van Goor, Harry ;

Eddy, Allison A. .

JOURNAL OF CLINICAL INVESTIGATION, 2008, 118 (11) :3522-3530

[25] Effect of autologous bone-marrow cell transplantation on ischemic ulcer in patients with Buerger's disease [J].

Saito, Yutaka ;

Sasaki, Ken-ichiro ;

Katsuda, Yousuke ;

Murohara, Toyoaki ;

Takeshita, Yoshiaki ;

Okazaki, Teiji ;

Arima, Ken ;

Katsuki, Yoshio ;

Shintani, Satoshi ;

Shimada, Toshifumi ;

Akashi, Hidetoshi ;

Ikeda, Hisao ;

Imaizumi, Tsutomu .

CIRCULATION JOURNAL, 2007, 71 (08) :1187-1192

[26] Therapeutic Lymphangiogenesis With Implantation of Adipose-Derived Regenerative Cells [J].

Shimizu, Yuuki ;

Shibata, Rei ;

Shintani, Satoshi ;

Ishii, Masakazu ;

Murohara, Toyoaki .

JOURNAL OF THE AMERICAN HEART ASSOCIATION, 2012, 1 (04)

[27] Mobilization of endothelial progenitor cells in patients with acute myocardial infarction [J].

Shintani, S ;

Murohara, T ;

Ikeda, H ;

Ueno, T ;

Honma, T ;

Katoh, A ;

Sasaki, KI ;

Shimada, T ;

Oike, Y ;

Imaizumi, T .

CIRCULATION, 2001, 103 (23) :2776-2779

[28] Augmentation of postnatal neovascularization with autologous bone marrow transplantation [J].

Shintani, S ;

Murohara, T ;

Ikeda, H ;

Ueno, T ;

Sasaki, K ;

Duan, JL ;

Imaizumi, T .

CIRCULATION, 2001, 103 (06) :897-903

[29] Synergistic effect of combined intramyocardial CD34+ cells and VEGF2 gene therapy after MI [J].

Shintani S. ;

Kusano K. ;

Ii M. ;

Iwakura A. ;

Heyd L. ;

Curry C. ;

Wecker A. ;

Gavin M. ;

Ma H. ;

Kearney M. ;

Silver M. ;

Thorne T. ;

Murohara T. ;

Losordo D.W. .

Nature Clinical Practice Cardiovascular Medicine, 2006, 3 (Suppl 1) :S123-S128

[30] Macrophage Plasticity and Polarization in Liver Homeostasis and Pathology [J].

Sica, Antonio ;

Invernizzi, Pietro ;

Mantovani, Alberto .

HEPATOLOGY, 2014, 59 (05) :2034-2042

← 1 2 3 4 →