Enzyme-linked immunosorbent assay for the determination of 20(S)-protopanaxatriol

被引：20

作者：

Jung, DW ^{[1
]}

Lee, JM ^{[1
]}

Sung, CK ^{[1
]}

机构：

[1] Chonnam Natl Univ, Coll Pharm, Buk Gu, Gwangju 500757, South Korea

来源：

ANALYTICA CHIMICA ACTA | 2002年 / 462卷 / 02期

关键词：

ELISA; 20(S)-protopanaxatriol; polyclonal antibodies; ginsenoside F-1;

D O I：

10.1016/S0003-2670(02)00340-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) method has been developed for the determination of 20(S)-protopanaxatriol (PPT), one of the major aglycones of dammarane-type ginseng saponins. Polyclonal antibodies raised against ginsenoside F-1 (GF(1))-bovine serum albumin showed high reactivities to PPT and GF(1), whereas they exhibited minor or even no cross-reactivities to other ginsenosides and protopanaxadiol (0.19%). The working range of this method extends from 50 pg ml(-1) to 20 ng ml(-1) of PPT. The assay reported here has been validated against an HPLC technique using PPT-containing samples and was shown to correlate closely (gamma = 0.993). This ELISA could be a useful tool for the determination of PPT contained in biological fluids and plant materials. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：157 / 163

页数：7

共 14 条

[1]

Akao T, 1998, BIOL PHARM BULL, V21, P245, DOI 10.1248/bpb.21.245

[2] DIGOXIN-SPECIFIC ANTIBODIES [J].

BUTLER, VP ;

CHEN, JP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1967, 57 (01) :71-&

[3] Gas chromatographic-mass spectrometric determination of 20(S)-protopanaxadiol and 20(S)-protopanaxatriol for study on human urinary excretion of ginsenosides after ingestion of ginseng preparations [J].

Cui, JF ;

Bjorkhem, I ;

Eneroth, P .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1997, 689 (02) :349-355

[4] ANALYSIS OF GINSENOSIDES BY CHROMATOGRAPHY AND MASS-SPECTROMETRY - RELEASE OF 20 S-PROTOPANAXADIOL AND 20 S-PROTOPANAXATRIOL FOR QUANTITATION [J].

CUI, JF ;

GARLE, M ;

LUND, E ;

BJORKHEM, I ;

ENEROTH, P .

ANALYTICAL BIOCHEMISTRY, 1993, 210 (02) :411-417

[5] AN ENZYME-IMMUNOASSAY FOR THE DETERMINATION OF TAXOL AND TAXANES IN TAXUS SP TISSUES AND HUMAN PLASMA [J].

GROTHAUS, PG ;

RAYBOULD, TJG ;

BIGNAMI, GS ;

LAZO, CB ;

BYRNES, JB .

JOURNAL OF IMMUNOLOGICAL METHODS, 1993, 158 (01) :5-15

[6] Main Ginseng saponin metabolites formed by intestinal bacteria [J].

Hasegawa, H ;

Sung, JH ;

Matsumiya, S ;

Uchiyama, M .

PLANTA MEDICA, 1996, 62 (05) :453-457

[7] REVERSAL OF DAUNOMYCIN AND VINBLASTINE RESISTANCE IN MULTIDRUG-RESISTANT P388 LEUKEMIA IN-VITRO THROUGH ENHANCED CYTOTOXICITY BY TRITERPENOIDS [J].

HASEGAWA, H ;

SUNG, JH ;

MATSUMIYA, S ;

UCHIYAMA, M ;

INOUYE, Y ;

KASAI, R ;

YAMASAKI, K .

PLANTA MEDICA, 1995, 61 (05) :409-413

[8]

Jung DW, 1998, CHEM PHARM BULL, V46, P1140, DOI 10.1248/cpb.46.1140

[9] STUDIES ON THE ENZYME-IMMUNOASSAY OF BIOACTIVE CONSTITUENTS IN ORIENTAL MEDICINAL DRUGS .6. ENZYME-IMMUNOASSAY OF GINSENOSIDE RB1 FROM PANAX-GINSENG [J].

KANAOKA, M ;

KATO, H ;

SHIMADA, F ;

YANO, S .

CHEMICAL & PHARMACEUTICAL BULLETIN, 1992, 40 (02) :314-317

[10] In vitro antigenotoxic activity of novel ginseng saponin metabolites formed by intestinal bacteria [J].

Lee, BH ;

Lee, SJ ;

Hui, JH ;

Lee, S ;

Sung, JH ;

Huh, JD ;

Moon, CK .

PLANTA MEDICA, 1998, 64 (06) :500-503

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