A modular tyrosine kinase deoxyribozyme with discrete aptamer and catalyst domains

被引：8
作者：
Dokukin, Victor [1 ]
Silverman, Scott K. [1 ]
机构：
[1] Univ Illinois, Dept Chem, Urbana, IL 61801 USA
来源：
CHEMICAL COMMUNICATIONS | 2014年 / 50卷 / 66期
基金：
美国国家卫生研究院; 美国国家科学基金会;
关键词：
IN-VITRO EVOLUTION; ROLLING CIRCLE AMPLIFICATION; PHOSPHORYLATING DNA; SIGNALING APTAMERS; RNA; RIBOZYME; ENZYMES; SENSORS; ATP; SPECIFICITY;
D O I：
10.1039/c4cc04253k
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We assess the utility of integrating a predetermined aptamer DNA module adjacent to a random catalytic DNA region for identifying new deoxyribozymes by in vitro selection. By placing a known ATP aptamer next to an N-40 random region, an explicitly modular DNA catalyst for tyrosine side chain phosphotylation is identified. The results have implications for broader identification of deoxyribozymes that function with small-molecule substrates.
引用
收藏
页码：9317 / 9320
页数：4
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