Adoptive Immunotherapy With Antigen-Specific T Cells During Extracorporeal Membrane Oxygenation (ECMO) for Adenovirus-Related Respiratory Failure in a Child Given Haploidentical Stem Cell Transplantation

被引：28

作者：

Di Nardo, Matteo ^{[1
]}

Li Pira, Giuseppina ^{[2
]}

Amodeo, Antonio ^{[3
]}

Cecchetti, Corrado ^{[1
]}

Giorda, Ezio ^{[4
]}

Ceccarelli, Stefano ^{[2
]}

Brescia, Letizia P. ^{[2
]}

Pirozzi, Nicola

Rutella, Sergio ^{[2
]}

Locatelli, Franco ^{[2
]}

Bertaina, Alice ^{[2
]}

机构：

[1] IRCCS Bambino Gesu Childrens Hosp, Dept Pediat Intens Care, I-00165 Rome, Italy

[2] IRCCS Bambino Gesu Childrens Hosp, Dept Pediat Hematol Oncol & Transfus Med, I-00165 Rome, Italy

[3] IRCCS Bambino Gesu Childrens Hosp, Dept Cardiac Surg, I-00165 Rome, Italy

[4] IRCCS Bambino Gesu Childrens Hosp, Core Res Facil, I-00165 Rome, Italy

来源：

PEDIATRIC BLOOD & CANCER | 2014年 / 61卷 / 02期

关键词：

adenovirus; ECMO; immune recovery; immunotherapy; stem cell transplantation; T lymphocyte; INFECTION; THROMBOCYTOPENIA; LYMPHOCYTES; DIAGNOSIS; CD4(+); CD8(+); VIRUS;

D O I：

10.1002/pbc.24753

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We report on the successful infusion of human adenovirus (HAdV)-specific T cells in a child with congenital amegakaryocytic thrombocytopenia, given T-cell-depleted hematopoietic stem cell transplantation (HSCT) from the HLA-haploidentical mother during extracorporeal membrane oxygenation (ECMO) for severe HAdV-related respiratory failure. Donor-derived, interferon (IFN)--secreting HAdV-specific T cells were enriched using the cytokine capture assay, after in vitro stimulation with overlapping peptides from the immunodominant HAdV5 hexon protein. Two weeks after T-cell transfer, viral load decreased and ECMO was discontinued. T-cell responses to HAdV antigens were documented after four weeks and were associated with viral clearance, immune reconstitution and clinical amelioration. Pediatr Blood Cancer 2014;61:376-379. (c) 2013 Wiley Periodicals, Inc.

引用

页码：376 / 379

页数：4

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