Phenotypical and morphological changes in the thymic microenvironment from ageing mice

被引：38
作者：
Aw, Danielle [1 ,2 ]
Taylor-Brown, Frances [1 ,2 ]
Cooper, Kate [1 ,2 ]
Palmer, Donald B. [1 ,2 ]
机构：
[1] Univ London Royal Vet Coll, Dept Vet Basic Sci Infect & Immun, London NW1 0TU, England
[2] Univ London Royal Vet Coll, Genes & Dev Grp, London NW1 0TU, England
来源：
BIOGERONTOLOGY | 2009年 / 10卷 / 03期
基金：
英国生物技术与生命科学研究理事会;
关键词：
Thymic involution; Thymic epithelial cells; Cytokeratin; EPITHELIAL-CELLS; LYMPHOHEMATOPOIETIC PROGENITORS; MURINE THYMUS; EXPRESSION; DIFFERENTIATION; INVOLUTION; REGENERATION; THYMOCYTE; MOUSE; IMMUNOSENESCENCE;
D O I：
10.1007/s10522-008-9182-2
中图分类号：
R592 [老年病学]; C [社会科学总论];
学科分类号：
03 ; 0303 ; 100203 ;
摘要：
The thymus is crucial for T-cell output and the age-associated involution of this organ, is thought to have a major impact in the decline in immunity that is seen in later life. The mechanism that underlines thymic involution is not known, however, we have evidence to suggest that this is may be due to changes in the thymic microenvironment. To further test this hypothesis, we quantified the in situ changes to markers that identify cortical and medullary thymic epithelial cells. This analysis revealed an age-dependent decline in cortical and medullary markers together with an increase in Notch and Delta expression, in older mice, as judged by immunohistochemistry. This was accompanied by alterations of the archetypal staining patterns and three dimensional analysis revealed changes in the morphology of the thymic microenvironment. These studies suggest that there are age-associated alterations in the thymic microenvironment, which may therefore play a role in thymic involution.
引用
收藏
页码：311 / 322
页数：12
相关论文
共 49 条
[1]   Dependence of self-tolerance on TRAF6-directed development of thymic stroma [J].
Akiyama, T ;
Maeda, S ;
Yamane, S ;
Ogino, K ;
Kasai, M ;
Kajiura, F ;
Matsumoto, M ;
Inoue, J .
SCIENCE, 2005, 308 (5719) :248-251
[2]   Lymphostromal interactions in thymic development and function [J].
Anderson, G ;
Jenkinson, EJ .
NATURE REVIEWS IMMUNOLOGY, 2001, 1 (01) :31-40
[3]   Generating intrathymic microenvironments to establish T-cell tolerance [J].
Anderson, Graham ;
Lane, Peter J. L. ;
Jenkinson, Eric J. .
NATURE REVIEWS IMMUNOLOGY, 2007, 7 (12) :954-963
[4]   Thymic atrophy in the mouse is a soluble problem of the thymic environment [J].
Aspinall, R ;
Andrew, D .
VACCINE, 2000, 18 (16) :1629-1637
[5]   Architectural changes in the thymus of aging mice [J].
Aw, Danielle ;
Silva, Alberto B. ;
Maddick, Mandy ;
von Zglinicki, Thomas ;
Palmer, Donald B. .
AGING CELL, 2008, 7 (02) :158-167
[6]   Immunosenescence: emerging challenges for an ageing population [J].
Aw, Danielle ;
Silva, Alberto B. ;
Palmer, Donald B. .
IMMUNOLOGY, 2007, 120 (04) :435-446
[7]   A SERIES OF 14 NEW MONOCLONAL-ANTIBODIES TO KERATINS - CHARACTERIZATION AND VALUE IN DIAGNOSTIC HISTOPATHOLOGY [J].
BARTEK, J ;
VOJTESEK, B ;
STASKOVA, Z ;
BARTKOVA, J ;
KEREKES, Z ;
REJTHAR, A ;
KOVARIK, J .
JOURNAL OF PATHOLOGY, 1991, 164 (03) :215-224
[8]   Phenotypic and immunohistological analyses of the human adult thymus: Evidence for an active thymus during adult life [J].
Bertho, JM ;
Demarquay, C ;
Moulian, N ;
VanderMeeren, A ;
Berrih-Aknin, S ;
Gourmelon, P .
CELLULAR IMMUNOLOGY, 1997, 179 (01) :30-40
[9]   Expression of JAGGED1 in T-lymphocytes results in thymic involution by inducing apoptosis of thymic stromal epithelial cells [J].
Beverly, L. J. ;
Ascano, J. M. ;
Capobianco, A. J. .
GENES AND IMMUNITY, 2006, 7 (06) :476-486
[10]   One for all and all for one: thymic epithelial stem cells and regeneration [J].
Blackburn, CC ;
Manley, NR ;
Palmer, DB ;
Boyd, RL ;
Anderson, G ;
Ritter, MA .
TRENDS IN IMMUNOLOGY, 2002, 23 (08) :391-395
12345