miR-378 and its host gene Ppargc1β exhibit independent expression in mouse skeletal muscle

被引:6
作者
Kang, Lin [1 ]
Han, Chunmiao [2 ,3 ]
Yang, Guangyan [1 ]
Li, Hu [2 ,3 ]
Li, Tingting [4 ]
Yang, Shu [1 ]
Liang, Na [2 ,3 ]
Zhong, Ran [2 ,3 ]
Jia, Lijing [1 ]
Zhu, Dahai [2 ,3 ,5 ]
Zhang, Yong [2 ,3 ]
机构
[1] Jinan Univ, Shenzhen Peoples Hosp, Dept Endocrinol, Clin Med Coll 2, Shenzhen 518020, Peoples R China
[2] Chinese Acad Med Sci, Inst Basic Med Sci, State Key Lab Med Mol Biol, Beijing 100005, Peoples R China
[3] Peking Union Med Coll, Sch Basic Med, Beijing 100005, Peoples R China
[4] Peking Univ, Sch Basic Med Sci, Dept Biomed Informat, Hlth Sci Ctr, Beijing 100191, Peoples R China
[5] Guangzhou Regenerat Med & Hlth Guangdong Lab, Guangzhou 510530, Peoples R China
基金
中国国家自然科学基金;
关键词
intronic microRNA; miR-378; Ppargc1; beta; transcriptional regulation; skeletal muscle; MICRORNAS; RNA; IDENTIFICATION; TRANSCRIPTION; HOMEOSTASIS; BIOGENESIS; TARGETS; BINDING; MIRNAS; SITES;
D O I
10.1093/abbs/gmaa061
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
MicroRNAs (miRNAs) are implicated in multiple biological processes in physiological and pathological settings. Nearly half of the known miRNAs are classified as 'intronic' miRNAs because they are embedded within the introns of protein-coding or noncoding genes. Such miRNAs were thought to be processed from primary host gene transcripts and share the promoter of their host. Recent analyses predicted that some intronic miRNAs might be transcribed and regulated as independent units, but there is little direct evidence for this in a specific biological context. Here, we focused on miR-378, which is located within the first intron of the peroxisome proliferator-activated receptor. coactivator 1-beta (Ppargc1 beta) gene and critically regulates skeletal muscle cell differentiation and muscle regeneration. We demonstrate that miR-378 and Ppargc1 beta exhibit distinct expression patterns during skeletal muscle cell differentiation. In terminally differentiated adult skeletal muscle tissues of mice, miR-378 is predominantly expressed in glycolytic muscle, whereas Ppargc1 beta is mainly expressed in oxidative soleus muscle. Mechanistically, miR-378, but not Ppargc1 beta, is regulated by the transcription factor, MyoD, in muscle cells. Our findings identify a regulatory model of miR-378 expression, thereby helping us to understand its physiological function in skeletal muscle.
引用
收藏
页码:883 / 890
页数:8
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