Inhibition of CD95-mediated apoptosis through β1 integrin in the HSG epithelial cell line

被引:5
作者
Dang, Howard
Dehghan, Parastou Lizeth
Goodwiler, Kai
Chen, Shuo
Zardeneta, Gustavo
Zhang, Bin-Xian
Yeh, Chih-Ko
机构
[1] Univ Texas, Hlth Sci Ctr, Dept Community Dent, San Antonio, TX 78229 USA
[2] Univ Texas, Hlth Sci Ctr, Dept Pediat Dent, San Antonio, TX 78229 USA
[3] Univ Texas, Hlth Sci Ctr, Dept Oral & Maxillofacial Surg, San Antonio, TX 78229 USA
[4] Univ Texas, Hlth Sci Ctr, Dept Med, San Antonio, TX 78229 USA
[5] Univ Texas, Hlth Sci Ctr, Dept Dent Diagnost Sci, San Antonio, TX 78229 USA
[6] S Texas Vet Hlth Care Syst, Audie L Murphy Div, Res Serv, San Antonio, TX USA
[7] S Texas Vet Hlth Care Syst, Ctr Geriatr Res Educ & Clin, San Antonio, TX USA
关键词
salivary gland; Bcl-2; apoptosis; CD95; integrins; p63;
D O I
10.1080/15419060600848532
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The HSG cell line serves as a model for salivary gland epithelial progenitor cell differentiation. In order for a progenitor cell to differentiate, the cell must maintain viability within its niche. Studies were designed to elucidate the mechanism for integrin-mediated HSG cell survival. HSG cells, grown on Matrigel(R), were resistant to CD95-mediated apoptosis. Western blot analysis showed that Matrigel(R) induced the expression of bcl-2, bcl-xL, p63, and Delta Np63. This induction occurred by as early as 2 hrs and remained for 24 hrs. CD95-mediated apoptosis resistance was dependent, however, upon the expression of the bcl-2 family. Furthermore, Matrigel(R) induced bcl-2 family expression was dependent on the transactivation of the EGF receptor pathway since PD98059 and AG1478 inhibited Matrigel(R) induced bcl-2 family expression and caused HSG cells to be sensitive to CD95-mediated apoptosis. Activation of the EGF receptor pathway, by itself, however, was not sufficient to inhibit apoptosis. Blocking antibody showed that bcl-2 family expression was mediated through beta 1 integrin. These studies show that salivary progenitor epithelial cell survival is integrin dependent and involves the transactivation of the EGF receptor pathway.
引用
收藏
页码:223 / 232
页数:10
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