ACT-PRESTO: Rapid and consistent tissue clearing and labeling method for 3-dimensional (3D) imaging

被引：162

作者：

Lee, Eunsoo ^{[1
,2
]}

Choi, Jungyoon ^{[1
,2
]}

Jo, Youhwa ^{[1
,2
]}

Kim, Joo Yeon ^{[1
,2
]}

Jang, Yu Jin ^{[3
]}

Lee, Hye Myeong ^{[3
]}

Kim, So Yeun ^{[4
]}

Lee, Ho-Jae ^{[5
]}

Cho, Keunchang ^{[5
]}

Jung, Neoncheol ^{[5
]}

Hur, Eun Mi ^{[6
,7
]}

Jeong, Sung Jin ^{[3
]}

Moon, Cheil ^{[4
]}

Choe, Youngshik ^{[3
]}

Rhyu, Im Joo ^{[1
,2
]}

Kim, Hyun ^{[1
,2
]}

Sun, Woong ^{[1
,2
]}

机构：

[1] Korea Univ, Coll Med, Dept Anat, Seoul 136, South Korea

[2] Korea Univ, Coll Med, Div Brain Korea Plus Program Biomed Sci 21, Seoul 136, South Korea

[3] Korea Brain Res Inst, Dept Neural Dev & Dis, Taegu 701300, South Korea

[4] DGIST, Grad Sch, Dept Brain & Cognit Sci, Taegu, South Korea

[5] Logos Biosyst Inc, Anyang Si 431755, Gyunggi Do, South Korea

[6] Korea Inst Sci & Technol, Brain Sci Inst, Ctr Neurosci, Seoul, South Korea

[7] Korea Univ Sci & Technol UST, Dept Neurosci, Daejeon, South Korea

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

基金：

新加坡国家研究基金会;

关键词：

SINGLE-CELL RESOLUTION; MOUSE-BRAIN; TOMOGRAPHY; AGENT;

D O I：

10.1038/srep18631

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Understanding the structural organization of organs and organisms at the cellular level is a fundamental challenge in biology. This task has been approached by reconstructing three-dimensional structure from images taken from serially sectioned tissues, which is not only labor-intensive and time-consuming but also error-prone. Recent advances in tissue clearing techniques allow visualization of cellular structures and neural networks inside of unsectioned whole tissues or the entire body. However, currently available protocols require long process times. Here, we present the rapid and highly reproducible ACT-PRESTO (active clarity technique-pressure related efficient and stable transfer of macromolecules into organs) method that clears tissues or the whole body within 1 day while preserving tissue architecture and protein-based signals derived from endogenous fluorescent proteins. Moreover, ACT-PRESTO is compatible with conventional immunolabeling methods and expedites antibody penetration into thick specimens by applying pressure. The speed and consistency of this method will allow high-content mapping and analysis of normal and pathological features in intact organs and bodies.

引用

页数：13

共 39 条

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