Micronucleus assay for predicting side effects of radiotherapy for cervical cancer

被引：10
|
作者：
da Silva, Edvane Borges [1 ]
Cavalcanti, Mariana Brayner [2 ]
Ferreira Da Silva, Catia Simone [1 ]
de Salazar e Fernandes, Thiago [3 ]
Melo, Jonathan Azevedo [4 ]
Lucena, Luciano [2 ]
Maciel Netto, Andre [2 ]
Amaral, Ademir [2 ]
机构：
[1] Univ Fed Pernambuco, Acad Ctr Vitoria, Recife, PE, Brazil
[2] Univ Fed Pernambuco, Dept Nucl Energy, Recife, PE, Brazil
[3] Univ Fed Pernambuco, Dept Biophys & Radiobiol, Recife, PE, Brazil
[4] Royal Portuguese Hosp, Royal Inst Radiotherapy, Recife, PE, Brazil
来源：
BIOTECHNIC & HISTOCHEMISTRY | 2021年 / 96卷 / 01期
关键词：
Cervical cancer; cytokinesis-block micronucleus; human; micronucleus; predictive test; radiotherapy; PERIPHERAL-BLOOD LYMPHOCYTES; EXPRESSION LEVELS; RISK-FACTORS; RADIATION; RADIOSENSITIVITY; CARCINOMA; FREQUENCY; ONCOLOGY;
D O I：
10.1080/10520295.2020.1759143
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Radiotherapy (RT) is an important treatment for cervical cancer. The quality of life of patients undergoing RT may be compromised during and following treatment by nausea, diarrhea, vomiting, burns, erythema and fistula. Cytokinesis-block micronucleus (CBMN) assays may be useful for predicting adverse effects of RT for cancer. The CBMN test is easy to perform and is reproducible for screening subjects exposed to ionizing radiation. We investigated the use of the frequency of micronuclei (MN) from peripheral blood samples, irradiated in vitro, as a possible biomarker to predict the side effects of RT in patients with cervical cancer. We used 10 patients with cervical cancer receiving RT and chemotherapy. We found a strong relation between the frequency of MN and the appearance of acute side effects of RT for cervical cancer. We suggest that the methodology presented here may be useful for predicting side effects of RT for patients affected by cervical cancer and who have undergone chemotherapy.
引用
收藏
页码：60 / 66
页数：7
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