Anticancer Efficacy and Mechanism of Amentoflavone for Sensitizing Oral Squamous Cell Carcinoma to Cisplatin

被引：17

作者：

Chen, Cheng-Hsien ^{[1
]}

Huang, Yi-Chi ^{[2
]}

Lee, Yuan-Hao ^{[3
]}

Tan, Zhao-Lin ^{[4
]}

Tsai, Chia-Jung ^{[5
]}

Chuang, Yao-Chen ^{[6
,7
]}

Tu, Hsi-Feng ^{[8
,9
]}

Liu, Ta-Chih ^{[10
]}

Hsu, Fei-Ting ^{[4
]}

机构：

[1] Show Chwan Mem Hosp, Surg Dept, Changhua, Taiwan

[2] Asia Univ Hosp, Dept Radiol, Taichung, Taiwan

[3] Univ Texas MD Anderson Canc Ctr, Dept Radiat Phys, Div Radiat Oncol, Houston, TX 77030 USA

[4] China Med Univ, Dept Biol Sci & Technol, Taichung, Taiwan

[5] I Shou Univ, Dept Med Imaging & Radiol Sci, Kaohsiung, Taiwan

[6] Macau Univ Sci & Technol, Fac Chinese Med, Macau, Peoples R China

[7] Kiang Wu Nursing Coll Macau, Macau, Peoples R China

[8] Natl Yang Ming Univ Hosp, Dept Dent, Yilan, Taiwan

[9] Natl Yang Ming Univ, Dent Sch, Dept Dent, Taipei, Taiwan

[10] Chang Bing Show Chwan Mem Hosp, Dept Internal Med, Div Hematol Oncol, Changhua, Taiwan

来源：

ANTICANCER RESEARCH | 2020年 / 40卷 / 12期

关键词：

Amentoflavone; cisplatin; oral squamous cell carcinoma; NF-kappa B; apoptosis; APOPTOSIS; COMBINATION; SUPPRESSION; RADIATION; CANCER; AIF;

D O I：

10.21873/anticanres.14695

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background/Aim: Nuclear factor kappa B (NF-kappa B) inactivation and apoptosis activation have been shown to enhance the anticancer effect of cisplatin in oral squamous cell carcinoma (OSCC). Amentoflavone may suppress NF-kappa B activity and trigger apoptosis in different types of cancer. The aim of this study was to investigate the anticancer effect and mechanism of amentoflavone in combination with cisplatin in OSCC. Materials and Methods: We investigated the combination effect and mechanism of amentoflavone and cisplatin via cell viability analysis, flow cytometry-based apoptosis analyses, transwell migration/invasion assay, immunofluorescence staining and western blotting assay. Results: Both amentoflavone and QNZ (NF-kappa B inhibitor) significantly increased cisplatin-induced cytotoxicity. Amentoflavone reduced cisplatin-triggered NF-kappa B activity and enhanced cisplatin-induced intrinsic caspasedependent and independent apoptotic pathways. Moreover, amentoflavone augments cisplatin-suppressed invasion and migration ability of OSCC cells. Conclusion: Inactivation of NF kappa B and induction of apoptosis through intrinsic caspasedependent and independent apoptotic pathways are associated with amentoflavone enhanced anti-OSCC efficacy of cisplatin.

引用

页码：6723 / 6732

页数：10

共 37 条

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