Sterilizing Bacillus pumilus spores using supercritical carbon dioxide

被引：85

作者：

Zhang, Jian

Burrows, Sarah

Gleason, Courtney

Matthews, Michael A. ^{[1
]}

Drews, Michael J.

LaBerge, Martine

An, Yuehuei H.

机构：

[1] Univ S Carolina, Dept Chem Engn, Columbia, SC 29208 USA

[2] Clemson Univ, Sch Mat Sci & Engn, Clemson, SC 29634 USA

[3] Clemson Univ, Dept Bioengn, Clemson, SC 29634 USA

[4] Med Univ S Carolina, Orthopaed Res Labs, Charleston, SC 29403 USA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2006年 / 66卷 / 03期

关键词：

sterilization; spores; supercritical carbon dioxide;

D O I：

10.1016/j.mimet.2006.01.012

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Supercritical carbon dioxide (SC CO2) has been evaluated as a new sterilization technology. Results are presented on killing of B. punfilus spores using SC CO2 containing trace levels of additives. Complete killing was achieved with 200 part per million (ppm) hydrogen peroxide in SC CO2 at 60 degrees C, 27.5 MPa. Addition of water to SC CO2 resulted in greater than three-log killing, but this is insufficient to claim sterilization. Neither ethanol nor isopropanol when added to SC CO2 affected killing. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：479 / 485

页数：7

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