Label-free and enzyme-free sensitive fluorescent detection of human immunodeficiency virus deoxyribonucleic acid based on hybridization chain reaction

被引：23
作者：
Guo, Qingquan [1 ]
Chen, Ying [1 ]
Song, Zhiping [1 ]
Guo, Liangqia [1 ]
Fu, Fengfu [1 ]
Chen, Guonan [1 ]
机构：
[1] Fuzhou Univ, Coll Chem, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Minist Educ,Key Lab Anal & Detect Food Safety, Fuzhou 350116, Peoples R China
来源：
ANALYTICA CHIMICA ACTA | 2014年 / 852卷
关键词：
Hybridization chain reaction; Human immunodeficiency virus; G-quadruplexes; N-methyl mesoporphyrin IX; QUADRUPLEX DNAZYME NANOWIRES; DNA DETECTION; NUCLEIC-ACIDS; ULTRASENSITIVE DETECTION; REACTION AMPLIFICATION; COLORIMETRIC DETECTION; AMPLIFIED DETECTION; ASSAY; POLYMERASE; UNIVERSAL;
D O I：
10.1016/j.aca.2014.09.032
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A label-free and enzyme-free sensitive fluorescent detection of human immunodeficiency virus (HIV) deoxyribonucleic acid (DNA) based on isothermal hybridization chain reaction (HCR) was developed. A G-quadruplex sequence which was incorporated into one of the two hairpin probes was inactive in the absence of target DNA. However, at the presence of target DNA numerous G-quadruplexes along DNA nanowires were self-assembled through HCR. Using N-methyl mesoporphyrin IX (NMM) as the fluorophore, a "turn-on" fluorescent response would be achieved and detected as low as 0.5 nmol L (1) of HIV DNA. This proposed method was applied to detect HIV DNA in biologic samples with satisfactory results. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码：244 / 249
页数：6
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