The analysis of thyroxine in human serum by an 'exact matching' isotope dilution method with liquid chromatography/tandem mass spectrometry

被引：38
作者：
Hopley, CJ
Stokes, P
Webb, KS
Baynham, M
机构：
[1] Lab Govt Chemist, Teddington TW11 0LY, Middx, England
[2] Appl Biosyst Inc, Warrington WA3 7QH, Cheshire, England
来源：
RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2004年 / 18卷 / 10期
关键词：
D O I：
10.1002/rcm.1441
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A method for the analysis of thyroxine in human serum, utilising 'exact matching' isotope dilution mass spectrometry (IDMS) in combination with liquid chromatography/tandem mass spectrometry (LC/MS/MS), has been developed with a limit of quantification of 0.5 ng g(-1) of thyroxine in human serum. The extraction and clean-up of thyroxine from serum involves an efficient protein-precipitation stage followed by a solid-phase extraction procedure to produce an extract essentially free from interfering compounds. The method is reproducible, with expanded uncertainties of less than 2%, and is relatively fast to perform. Copyright (C) 2004 John Wiley Sons, Ltd.
引用
收藏
页码：1033 / 1038
页数：6
相关论文
共 11 条
[1]  
Bievre PD, 1996, ACCREDIT QUAL ASSUR, V1, P3, DOI [10.1007/s007690050026, DOI 10.1007/S007690050026]
[2]  
De Brabandere VI, 1998, RAPID COMMUN MASS SP, V12, P1099, DOI 10.1002/(SICI)1097-0231(19980831)12:16<1099::AID-RCM290>3.0.CO
[3]  
2-J
[4]   REDUCTION OF SYSTEMATIC-ERRORS IN QUANTITATIVE-ANALYSIS BY ISOTOPE-DILUTION MASS-SPECTROMETRY (IDMS) - AN ITERATIVE METHOD [J].
HENRION, A .
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 1994, 350 (12) :657-658
[5]  
MOLLER B, 1983, CLIN CHEM, V29, P2106
[6]   DEVELOPMENT OF A GAS-CHROMATOGRAPHIC SELECTED ION MONITORING ASSAY FOR THYROXINE (T4) IN HUMAN-SERUM [J].
RAMSDEN, DB ;
FARMER, MJ .
BIOMEDICAL MASS SPECTROMETRY, 1984, 11 (08) :421-427
[7]   Rapid HPLC analysis of thyroid gland hormones tri-iodothyronine (T3) and thyroxine (T4) in human biological fluids after SPE [J].
Samanidou, VF ;
Gika, HG ;
Papadoyannis, IN .
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2000, 23 (05) :681-692
[8]  
Sargent M., 2002, Guidelines for Achieving High Accuracy in Isotope Dilution Mass Spectrometry
[9]   MEASUREMENT OF THYROXINE IN HUMAN-SERUM BY ISOTOPE-DILUTION MASS-SPECTROMETRY - DEFINITIVE METHODS IN CLINICAL-CHEMISTRY .5. [J].
SIEKMANN, L .
BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY, 1987, 14 (11) :683-688
[10]  
Tai SSC, 2002, CLIN CHEM, V48, P637
12