Heme oxygenase-1 mediates the inhibitory actions of brazilin in RAW264.7 macrophages stimulated with lipopolysaccharide

被引：53

作者：

Hu, Chien-Ming ^{[1
]}

Liu, Yung-Hung ^{[1
]}

Cheah, Khoot-Peng ^{[1
]}

Li, Joe-Sharg ^{[1
]}

Lam, Carlos-Shu Kei ^{[2
]}

Yu, Wen-Yu ^{[1
]}

Choy, Cheuk-Sing ^{[1
,3
]}

机构：

[1] Taipei Med Univ Hosp, Emergency Dept, Taipei 110, Taiwan

[2] Taipei Med Univ, Municipal Wan Fang Hosp, Emergency Dept, Taipei, Taiwan

[3] Taipei Med Univ Hosp, Sch Publ Hlth, Taipei 110, Taiwan

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2009年 / 121卷 / 01期

关键词：

Brazilin; Heme oxygenase-1; Anti-inflammation; Lipopolysaccharide; Inducible nitric oxide synthase; Macrophages; NITRIC-OXIDE SYNTHASE; NF-KAPPA-B; OXIDATIVE STRESS; UP-REGULATION; ENDOTHELIAL-CELLS; NO PRODUCTION; IN-VITRO; EXPRESSION; BILIRUBIN; INJURY;

D O I：

10.1016/j.jep.2008.09.030

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Brazilin, the main constituent of Caesalpinia sappan L., is a natural red pigment that has been reported to possess anti-inflammatory properties. This study aimed to identify a novel anti-inflammatory mechanism of brazilin. We found that brazilin did not cause cytotoxicity below 300 mu M, and activated heme oxygenase-1 (HO-1) protein synthesis in a concentration-dependent manner at 10-300 mu M in RAW264.7 macrophages without affecting mRNA transcription of HO-1. Additionally, brazilin increased bilirubin production and HO-1 activity in RAW264.7 macrophages. In lipopolysaccharide (LPS)-stimulated macrophages, brazilin suppressed the release of nitric oxide (NO), prostaglandin E-2 (PGE(2)), interleukin (IL)-1 beta and tumor necrosis factor-alpha (TNF-alpha), and reduced the expression of inducible nitric oxide synthase (iNOS). A specific inhibitor of HO-1, Zn(II) protoporphyrin IX, blocked the suppression of NO production, cytokines release and iNOS expression by brazilin. These results suggest that brazilin possesses antiinflammatory actions in macrophages and works through a novel mechanism involving the action of HO-1. (c) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：79 / 85

页数：7

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