A Colloidal Gold Nanoparticle-Based Immunochromatographic Test Strip for Rapid and Convenient Detection of Staphylococcus Aureus

被引：28

作者：

Niu, Kaili ^{[1
,2
]}

Zheng, Xiaoping ^{[3
]}

Huang, Chusen ^{[1
,2
]}

Xu, Kuan ^{[1
,2
]}

Zhi, Yuan ^{[1
,2
]}

Shen, Hebai ^{[1
,2
]}

Jia, Nengqin ^{[1
,2
]}

机构：

[1] Shanghai Normal Univ, Educ Minist Key Lab Resource Chem, Dept Chem, Shanghai 200234, Peoples R China

[2] Shanghai Normal Univ, Shanghai Key Lab Rare Earth Funct Mat, Dept Chem, Shanghai 200234, Peoples R China

[3] China Minist Agr, Food Qual Supervis & Inspect Ctr, Shanghai 200234, Peoples R China

来源：

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2014年 / 14卷 / 07期

关键词：

Staphylococcus Aureus; Gold Nanoparticles; Immunochromatography; Test Strip; POLYMERASE-CHAIN-REACTION; ESCHERICHIA-COLI; ASSAY; IDENTIFICATION; BACTERIA;

D O I：

10.1166/jnn.2014.8703

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An immunochromatographic test strip using gold nanoparticles-staphylococcus aureus monoclonal antibody conjugates was developed for the rapid and convenient detection of staphylococcus aureus based on a double-antibody sandwich format. The detection limit and the detection rate of this test strip is 10(3) CFU /mL and 98.7%, respectively. It could be used for the rapid detection of staphylococcus aureus in food and the results can be visually identified by the naked eye within 10 min. Compared with conventional bacterial detection methods, this developed immunochromatographic assay based test strip has several advantages including simple, fast, low-cost, favorable sensitivity and specificity, exhibiting a great potential for application in food safety control systems and clinical diagnosis.

引用

页码：5151 / 5156

页数：6

共 28 条

[1] Enhanced Agglutination Reaction of ABO Subgroups by Gold Nanoparticle Solution: Implication for Identification of ABO Subgroups
Ammaranond, P.
Sriyarak, J.
Saejong, S.
Deesin, P.
Seereemaspun, A.
Rojanathanes, R.
[J]. JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 2011, 7 (06) : 840 - 845
[2] Detection of Tyrosine Hydroxylase in Dopaminergic Neuron Cell Using Gold Nanoparticles-Based Barcode DNA
An, Jeung Hee
Oh, Byung-Keun
Choi, Jeong Woo
[J]. JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 2013, 9 (04) : 639 - 643
[3] Avila E. M.-, 2012, J BIOMED NANOTECHNOL, V8, P991
[4] Evaluation of an immunochromatography strip assay for the detection of Salmonella sp from poultry
Bautista, DA
Elankumaran, S
Arking, JA
Heckert, RA
[J]. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2002, 14 (05) : 427 - 430
[5] DETECTION OF COLIFORM BACTERIA AND ESCHERICHIA-COLI BY MULTIPLEX POLYMERASE CHAIN-REACTION - COMPARISON WITH DEFINED SUBSTRATE AND PLATING METHODS FOR WATER-QUALITY MONITORING
BEJ, AK
MCCARTY, SC
ATLAS, RM
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (08) : 2429 - 2432
[6] The isolation and detection of Escherichia coli O157 by use of immunomagnetic separation and immunoassay procedures
Bennett, AR
MacPhee, S
Betts, RP
[J]. LETTERS IN APPLIED MICROBIOLOGY, 1996, 22 (03) : 237 - 243
[7] DETECTION OF LISTERIA SPECIES AND LISTERIA-MONOCYTOGENES USING POLYMERASE CHAIN-REACTION
BORDER, PM
HOWARD, JJ
PLASTOW, GS
SIGGENS, KW
[J]. LETTERS IN APPLIED MICROBIOLOGY, 1990, 11 (03) : 158 - 162
[8] FLUORESCENT DETECTION-POLYMERASE CHAIN-REACTION (FD-PCR) ASSAY ON MICROWELL PLATES AS A SCREENING-TEST FOR SALMONELLAS IN FOODS
CANO, RJ
RASMUSSEN, SR
FRAGA, GS
PALOMARES, JC
[J]. JOURNAL OF APPLIED BACTERIOLOGY, 1993, 75 (03): : 247 - 253
[9] Chang T. C., 1992, J RAPID METH AUT MIC, V1, P133
[10] EVALUATION OF A LATEX AGGLUTINATION-TEST FOR RAPID IDENTIFICATION OF STAPHYLOCOCCUS-AUREUS FROM FOODS
CHANG, TC
HUANG, SH
[J]. JOURNAL OF FOOD PROTECTION, 1993, 56 (09) : 759 - 762

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