Fluorescence-Amplifying Assay for Irradiated DNA Lesions Using Water-Soluble Conjugated Polymers

被引：9

作者：

Feng, Fude ^{[1
]}

Duan, Xinrui ^{[1
]}

Wang, Shu ^{[1
]}

机构：

[1] Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Organ Solids, Inst Chem, Beijing 100190, Peoples R China

来源：

MACROMOLECULAR RAPID COMMUNICATIONS | 2009年 / 30卷 / 03期

基金：

中国国家自然科学基金;

关键词：

conjugated polymers; DNA lesions; fluorescence; PYRIMIDINE DIMERS; CHEMICAL SENSORS; PHOTOPRODUCTS; PHOTOLYASE; QUANTITATION;

D O I：

10.1002/marc.200800543

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

A new platform has been developed for DNA lesion detection using a cationic conjugated polymer (CCP). DNA that contains two adjacent thymine bases is irradiated with ultraviolet light to allow for the formation of cyclobutane pyrimidine dimers and pyrimidine-pyrimidone dimers. The DNA lesions block the primer extension, and the base labeled with fluorescein cannot be incorporated into the DNA strand. Addition of the CCP leads to inefficient fluorescence resonance energy transfer (FRET) from CCP to fluorescein. For the case without DNA lesions, successful primer extension allows for efficient FRET between them. In view of the FRET signal changes, the DNA lesions can be detected. This new protocol offers a convenient detection for DNA lesions in aqueous solution without any isolation and washing steps.

引用

页码：147 / 151

页数：5

共 30 条

[1] CYCLOBUTANE PYRIMIDINE DIMERS AND (6-4) PHOTOPRODUCTS BLOCK POLYMERIZATION BY DNA-POLYMERASE-I [J].

CHAN, GL ;

DOETSCH, PW ;

HASELTINE, WA .

BIOCHEMISTRY, 1985, 24 (21) :5723-5728

[2] Individual determination of the yield of the main UV-induced dimeric pyrimidine photoproducts in DNA suggests a high mutagenicity of CC photolesions [J].

Douki, T ;

Cadet, J .

BIOCHEMISTRY, 2001, 40 (08) :2495-2501

[3] MEASUREMENT OF PYRIMIDINE(6-4) PHOTOPRODUCTS IN DNA BY A MILD ACIDIC HYDROLYSIS HPLC FLUORESCENCE DETECTION ASSAY [J].

DOUKI, T ;

VOITURIEZ, L ;

CADET, J .

CHEMICAL RESEARCH IN TOXICOLOGY, 1995, 8 (02) :244-253

[4] A sensitive and homogeneous SNP detection using cationic conjugated polymers [J].

Duan, Xinrui ;

Li, Zhengping ;

He, Fang ;

Wang, Shu .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (14) :4154-+

[5] Fluorescent conjugated polyelectrolyte as an indicator for convenient detection of DNA methylation [J].

Feng, Fude ;

Wang, Hongzhong ;

Han, Lingli ;

Wang, Shu .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (34) :11338-11343

[6] UV-INDUCED FORMATION OF PYRIMIDINE DIMERS IN NUCLEOSOME CORE DNA IS STRONGLY MODULATED WITH A PERIOD OF 10.3 BASES [J].

GALE, JM ;

NISSEN, KA ;

SMERDON, MJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (19) :6644-6648

[7] DNA detection using water-soluble conjugated polymers and peptide nucleic acid probes [J].

Gaylord, BS ;

Heeger, AJ ;

Bazan, GC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (17) :10954-10957

[8] QUANTITATION OF CYCLOBUTANE PYRIMIDINE DIMER FORMATION IN DOUBLE-STRANDED AND SINGLE-STRANDED-DNA FRAGMENTS OF DEFINED SEQUENCE [J].

GORDON, LK ;

HASELTINE, WA .

RADIATION RESEARCH, 1982, 89 (01) :99-112

[9] Fluorescent amplifying recognition for DNA G-quadruplex folding with a cationic conjugated polymer: A platform for homogeneous potassium detection [J].

He, F ;

Tang, YL ;

Wang, S ;

Li, YL ;

Zhu, DB .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (35) :12343-12346

[10] The XPC-HR23B complex displays high affinity and specificity for damaged DNA in a true-equilibrium fluorescence assay [J].

Hey, T ;

Lipps, G ;

Sugasawa, K ;

Iwai, S ;

Hanaoka, F ;

Krauss, G .

BIOCHEMISTRY, 2002, 41 (21) :6583-6587

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