Molecular properties and antioxidant activities of polysaccharides isolated from alkaline extract of wild Armillaria ostoyae mushrooms

被引：76

作者：

Siu, Ka-Chai ^{[1
]}

Xu, Lijian ^{[2
]}

Chen, Xia ^{[1
]}

Wu, Jian-Yong ^{[1
]}

机构：

[1] Hong Kong Polytech Univ, State Key Lab Chinese Med & Mol Pharmacol Shenzhe, Dept Appl Biol & Chem Technol, Kowloon, Hong Kong, Peoples R China

[2] Heilongjiang Univ, Coll Agr Resource & Environm, Harbin 15000, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2016年 / 137卷

关键词：

Armillaria ostoyae; Polysaccharides; Extraction; Fractionation; Structure; Antioxidant activity; WEIGHT POLYSACCHARIDES; ANTICANCER ACTIVITY; LENTINUS-EDODES; FRUITING BODIES; EXOPOLYSACCHARIDES; FRACTIONATION; PURIFICATION; DEGRADATION; COMPONENTS; ANTITUMOR;

D O I：

10.1016/j.carbpol.2015.05.061

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This study aims to discover novel and bioactive polysaccharides (PS) from wild Armillaria ostoyae, a honey mushroom species. Two PS designated AkPS1V-1 (66.6 kDa) and AkPS1V-2 (15.3 kDa) were isolated and fractionated by anion ion exchange (IEC) and size exclusion chromatography (SEC) from the alkaline extract of A. ostoyae mushrooms. AkPS1V-1 was a glucan composed of solely glucose residues and AkPS1V-2 a heteropolysaccharide composed of glucose and galactose at 6:1 molar ratio. AkPS1V-2 exhibited higher antioxidant activities than AkPS1V-1 based on reducing power, radical scavenging and metal chelating assays. The structure of AkPS1V-2 was further analyzed and elucidated as a branched galactoglucan with a backbone composed of (1 -> 6)-beta-D glucopyranosyl, (1 -> 3)-beta-D glucopyranosyl, (1 -> 3)-alpha-D-galactopyranosyl and (1 3,6)-beta-D-glucopyranosyl residues at 3:1:1:1 ratio, and side chain of (1 -> 3)-beta-D-glucopyranosyl residue. This is the first report on a pure PS structure and its antioxidant activities from this mushroom species. (C) 2015 Published by Elsevier Ltd.

引用

页码：739 / 746

页数：8

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