Molecular and Genomic Impact of Large and Small Lateral Dimension Graphene Oxide Sheets on Human Immune Cells from Healthy Donors

被引：82

作者：

Orecchioni, Marco ^{[1
]}

Jasim, Dhifaf A. ^{[2
]}

Pescatori, Mario ^{[1
,3
]}

Manetti, Roberto ^{[4
]}

Fozza, Claudio ^{[5
]}

Sgarrella, Francesco ^{[1
]}

Bedognetti, Davide ^{[6
]}

Bianco, Alberto ^{[7
]}

Kostarelos, Kostas ^{[2
]}

Delogu, Lucia Gemma ^{[1
]}

机构：

[1] Univ Sassari, Dept Chem & Pharm, I-07100 Sassari, Italy

[2] Univ Manchester, Fac Med & Human Sci, Nanomed Lab, Manchester M13 9PT, Lancs, England

[3] Heath E Solut, NL-3016 DL Rotterdam, Netherlands

[4] Univ Sassari, Dept Clin Med & Expt Oncol, I-07100 Sassari, Italy

[5] Univ Sassari, Dept Biomed Sci, I-07100 Sassari, Italy

[6] Sidra Med & Res Ctr, Res Branch, Doha, Qatar

[7] CNRS, Inst Biol Mol & Cellulaire, Lab Immunopathol & Chim Therapeut, F-67000 Strasbourg, France

来源：

ADVANCED HEALTHCARE MATERIALS | 2016年 / 5卷 / 02期

关键词：

FUNCTIONALIZED CARBON NANOTUBES; PRISTINE GRAPHENE; GENE-EXPRESSION; IN-VITRO; RESPONSES; NANOTECHNOLOGY; MACROPHAGES; BIOMEDICINE; TOXICOLOGY; NANOSHEETS;

D O I：

10.1002/adhm.201500606

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Graphene oxide (GO) is attracting great interest in biomedical sciences. The impact of GO on immune cells is one fundamental area of study that is often overlooked, but critical in terms of clinical translation. This work investigates the effects of two types of thoroughly characterized GO sheets, different in their lateral dimension, on human peripheral immune cells provided from healthy donors using a wide range of assays. After evaluation of cell viability, the gene expression was analyzed, following GO exposure on 84 genes related to innate and adaptive immune responses. Exposure to GO small sheets was found to have a more significant impact on immune cells compared to GO large sheets, reflected in the upregulation of critical genes implicated in immune responses and the release of cytokines IL1 beta and TNF alpha. These findings were further confirmed by whole-genome microarray analysis of the impact of small GO sheets on T cells and monocytes. Activation in both cell types was underlined by the overexpression of genes such as CXCL10 and receptor CXCR3. Significant energy-dependent pathway modulation was identified. These findings can potentially pave the foundations for further design of graphene that can be used for immune modulation applications, for example in cancer immunotherapy.

引用

页码：276 / 287

页数：12