The effect of amitraz poisoning on formation of methemoglobin and hemolytic anemia in guinea-pigs

This investigation was carried out to determine the effect of amitraz poisoning on the the formation of methemoglobin and osmotic fragility of erytrocytes. In this study, 12 guinea-pigs, weighing 250-300 gr were used. They were equally divided into two groups, control and experimental. The animals in the experimental group were intraperitoneally injected with amitraz at a dose of 1 mg/kg body weight. One hour after amitraz injections, erytrocyte, hematocrit, hemoglobin levels and also percent hemolysis of erytrocytes were measured in the blood samples taken from the control and experimental groups. In the experimental group, the value of hemoglobin was lower while the value of methemoglobin was higher than those of the control group (p<0.01). In this group it was also observed that the osmotic fragility of erytrocytes were decreased (p<0.01) though the small decreases in the values of erytrocytes and hematocrit were not statistically significant.