iTRAQ-based proteomic analysis identifies proteins involved in limb regeneration of swimming crab Portunus tritubercuLatus

被引：15

作者：

Fu, Yuanyuan ^{[1
,2
]}

Zhu, Fang ^{[1
,2
]}

Liu, Lei ^{[1
,2
]}

Lu, Shaokun ^{[1
,2
]}

Ren, Zhiming ^{[1
,2
]}

Mu, Changkao ^{[1
,2
]}

Li, Ronghua ^{[1
,2
]}

Song, Weiwei ^{[1
,2
]}

Shi, Ce ^{[1
,2
]}

Ye, Yangfang ^{[1
,2
]}

Wang, Chunlin ^{[1
,2
]}

机构：

[1] Ningbo Univ, Sch Marine Sci, Fenghua Rd 818, Ningbo 315211, Zhejiang, Peoples R China

[2] Collaborat Innovat Ctr Zhejiang Marine High Effic, Ningbo 315211, Zhejiang, Peoples R China

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS | 2018年 / 26卷

基金：

中国国家自然科学基金;

关键词：

Portunus trituberculatus; Swimming crab; iTRAQ; Limb regeneration; Proteomic analysis; ECM; RT-PCR; CUCUMBER APOSTICHOPUS-JAPONICUS; FIDDLER-CRAB; UCA-PUGILATOR; ERIOCHEIR-SINENSIS; BASEMENT-MEMBRANE; EPITHELIAL-CELLS; SKELETAL-MUSCLE; GROWTH; AUTOTOMY; PHOSPHORYLATION;

D O I：

10.1016/j.cbd.2018.02.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The swimming crab (Portunus tntuberculatm) has a striking capacity for limb regeneration, which has drawn the interest of many researchers.In this study, isobaric tag for relative and absolute quantitation (iTRAQ) approach was utilised to investigate protein abundance changes during limb regeneration in this species. A total of 1830 proteins were identified, of which 181 were significantly differentially expressed, with 94 upregulated and 87 downregulated. Our results highlight the complexity of limb regeneration and its regulation through cooperation of various biological processes including cytoskeletal changes, extracellular matrix (ECM) remodelling and ECM- receptor interactions, protein synthesis, signal recognition and transduction, energy production and conversion, and substance transport and metabolism. Additionally, real-time PCR confirmed that mRNA levels of differentially expressed genes were correlated with protein levels. Our results provide a basis for studying the regulatory mechanisms associated with crab limb regeneration.

引用

页码：10 / 19

页数：10

共 62 条

[1] Proteome and proteomics: New technologies, new concepts, and new words [J].