The effect of sodium alginate on the immune response of tiger shrimp via dietary administration: Activity and gene transcription

被引：57
作者：
Liu, Chun-Hung
Yeh, Shinn-Pyng
Kuo, Chin-Ming
Cheng, Winton [1 ]
Chou, Chang-Hung
机构：
[1] Natl Pingtung Univ Sci & Technol, Dept Aquaculture, Pingtung 91201, Taiwan
[2] Acad Sinica, Marine Res Stn, Inst Zool, Ilan 26242, Taiwan
[3] Natl Pingtung Univ Sci & Technol, Dept Trop Agr & Int Cooperat, Pingtung 91201, Taiwan
来源：
FISH & SHELLFISH IMMUNOLOGY | 2006年 / 21卷 / 04期
关键词：
Penaeus monodon; sodium alginate; dietary administration; immunogene; polymerase chain reaction; immunomodulator;
D O I：
10.1016/j.fsi.2006.02.003
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
The total haemocyte count (THC), phenoloxidase (PO) activity, respiratory bursts (release of superoxide anions), and superoxide dismutase (SOD) activity, as well as expressions of beta-1,3-glucan-binding protein (beta GBP), prophenoloxidase (proPO), peroxinectin (PE), cytosolic SOD (cyt-SOD), penaeidin-5 (PA-5), and a single whey acidic protein (WAP) domain protein (SWDP) gene were determined in the tiger shrimp Penaeus monodon (15.6-19.5 g) which had individually been fed diets containing sodium alginate at 0, 1.0, or 2.0 g kg(-1) for 5 months. Results showed that shrimp fed a diet containing 1.0 and 2.0 g kg(-1) sodium alginate had significantly increased SOD activity but decreased respiratory bursts. The expressions of beta GBP, PE, cyt-SOD, PA-5, and SWDP were significantly elevated in shrimp fed the <= 2.0 g kg(-1) sodium alginate-containing diet for 5 months. However, no significant differences in THC, PO activity, or proPO mRNA transcription in shrimp were observed among the three treatments. It was concluded that sodium alginate can be used as an immunomodulator for shrimp through dietary administration to modify immune genes expression of shrimp. (c) 2006 Elsevier Ltd. All rights reserved.
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收藏
页码：442 / 452
页数：11
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