Spatial transcriptomes of iron-deficient and cadmium-stressed rice

被引:56
作者
Ogo, Yuko [1 ,2 ]
Kakei, Yusuke [1 ,2 ]
Itai, Reiko Nakanishi [1 ,2 ]
Kobayashi, Takanori [1 ,2 ,3 ]
Nakanishi, Hiromi [1 ,2 ]
Takahashi, Hirokazu [4 ]
Nakazono, Mikio [4 ]
Nishizawa, Naoko K. [1 ,2 ,3 ]
机构
[1] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Global Agr Sci, Bunkyo Ku, Tokyo 1138657, Japan
[2] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Appl Biol Chem, Bunkyo Ku, Tokyo 1138657, Japan
[3] Ishikawa Prefectural Univ, Res Inst Bioresources & Biotechnol, Nonoichi, Ishikawa 9218836, Japan
[4] Nagoya Univ, Grad Sch Bioagr Sci, Lab Plant Genet & Breeding, Chikusa Ku, Nagoya, Aichi 4648601, Japan
关键词
cadmium (Cd); iron (Fe); laser microdissection; Oryza sativa (rice); transcriptome; ACQUISITION-RELATED GENES; MUGINEIC ACID; REAL-TIME; 2-DEOXYMUGINEIC ACID; EXPRESSION PROFILE; TRANSPORTER FAMILY; REDUCTASE-ACTIVITY; METAL TRANSPORTER; ACTS DOWNSTREAM; TRANSLOCATION;
D O I
10.1111/nph.12577
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Although the genes involved in metal homeostasis have been investigated over the past few decades, many genes related to metal homeostasis remain uncharacterized, and a comprehensive analysis of the expression of these genes is required. In the present study, we investigated the spatial gene expression profile of iron (Fe)-deficient and cadmium (Cd)-stressed Oryza sativa (rice) using laser microdissection and micro-array analysis. Roots of Fe-deficient and Cd-stressed rice were separated into the vascular bundle, cortex, and epidermis plus exodermis. In addition, vascular bundles from new and old leaves at the lowest node, which are important for metal distribution, were analyzed separately. The spatial expression patterns were distinct in each tissue type. Fe deficiency and Cd stress also had significant effects on the transcriptomes, although these were less pronounced than the spatial effects. Genes encoding transporters involved in metal homeostasis, proteins associated with heavy metal detoxification, and phytohormone-related proteins were comprehensively investigated. Additionally, cis motifs involved in the regulation of these diverse expression changes in various tissue types were predicted. The spatial transcriptomes presented here provide novel insight into the molecular mechanisms of metal homeostasis.
引用
收藏
页码:781 / 794
页数:14
相关论文
共 48 条
[41]   Synergistic inhibitory effect of selenium, iron, and humic acid on cadmium uptake in rice (Oryza sativa L.) seedlings in hydroponic culture [J].
Zhang, Hongyu ;
Xie, Shuyun ;
Bao, Zhengyu ;
Carranza, Emmanuel John M. ;
Tian, Huan ;
Wei, Changhua .
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2021, 28 (45) :64652-64665
[42]   Spatial ionomics provides new insights into the accumulation and transport of mineral ions in rice (Oryza sativa L.) under Cadmium stress [J].
Luo, Qiuhong ;
Bai, Bin ;
Xie, Yunhe ;
Yao, Dongping ;
Chen, Zhe ;
Zhang, Dongmeng ;
Liu, Yu ;
Xiao, Yinghui ;
Yu, Yinghong ;
Wu, Jun .
ENVIRONMENTAL AND EXPERIMENTAL BOTANY, 2023, 208
[43]   Influence of FeSO4, nano zero-valent iron, and their CaCO3 composites on the formation of iron plaque and cadmium translocation in rice (Oryza sativa L.) [J].
Xu, Bo ;
Chen, Jianyu ;
Hu, Yuanbiao ;
You, Yanlin ;
Sun, Xiaochun ;
Yu, Jinyong ;
Guo, Xiaodong ;
Hu, Cong ;
Chen, Chunle ;
Chen, Yanhui ;
Wang, Guo .
ENVIRONMENTAL POLLUTANTS AND BIOAVAILABILITY, 2024, 36 (01)
[44]   Effects of microbial organic fertilizer (MOF) application on cadmium uptake of rice in acidic paddy soil: Regulation of the iron oxides driven by the soil microorganisms [J].
Kong, Fanyi ;
Lu, Shenggao .
ENVIRONMENTAL POLLUTION, 2022, 307
[45]   Route and Regulation of Zinc, Cadmium, and Iron Transport in Rice Plants (Oryza sativa L.) during Vegetative Growth and Grain Filling: Metal Transporters, Metal Speciation, Grain Cd Reduction and Zn and Fe Biofortification [J].
Yoneyama, Tadakatsu ;
Ishikawa, Satoru ;
Fujimaki, Shu .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2015, 16 (08) :19111-19129
[46]   The bHLH protein OsIRO3 is critical for plant survival and iron (Fe) homeostasis in rice (Oryza sativa L.) under Fe-deficient conditions [J].
Wang, Fan ;
Itai, Reiko N. ;
Nozoye, Tomoko ;
Kobayashi, Takanori ;
Nishizawa, Naoko K. ;
Nakanishi, Hiromi .
SOIL SCIENCE AND PLANT NUTRITION, 2020, 66 (04) :579-592
[47]   Role of iron manganese plaque in the safe production of rice (Oryza sativa L.) grains: Field evidence at plot and regional scales in cadmium-contaminated paddy soils [J].
Kong, Fanyi ;
Zhou, Jiahang ;
Guan, Dong-Xing ;
Wu, Nei ;
Lu, Shenggao ;
Wang, Haizhen .
SCIENCE OF THE TOTAL ENVIRONMENT, 2023, 903
[48]   Synergistic effects of silicon and goethite co-application in alleviating cadmium stress in rice (Oryza sativa L.): Insights into plant growth and iron plaque formation mechanisms [J].
Dai, Zhaoyi ;
Yu, Lei ;
Ma, Pan ;
Wang, Yaojing ;
An, Shuai ;
Liu, Mingda .
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2024, 281