Spatial transcriptomes of iron-deficient and cadmium-stressed rice

被引:56
作者
Ogo, Yuko [1 ,2 ]
Kakei, Yusuke [1 ,2 ]
Itai, Reiko Nakanishi [1 ,2 ]
Kobayashi, Takanori [1 ,2 ,3 ]
Nakanishi, Hiromi [1 ,2 ]
Takahashi, Hirokazu [4 ]
Nakazono, Mikio [4 ]
Nishizawa, Naoko K. [1 ,2 ,3 ]
机构
[1] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Global Agr Sci, Bunkyo Ku, Tokyo 1138657, Japan
[2] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Appl Biol Chem, Bunkyo Ku, Tokyo 1138657, Japan
[3] Ishikawa Prefectural Univ, Res Inst Bioresources & Biotechnol, Nonoichi, Ishikawa 9218836, Japan
[4] Nagoya Univ, Grad Sch Bioagr Sci, Lab Plant Genet & Breeding, Chikusa Ku, Nagoya, Aichi 4648601, Japan
关键词
cadmium (Cd); iron (Fe); laser microdissection; Oryza sativa (rice); transcriptome; ACQUISITION-RELATED GENES; MUGINEIC ACID; REAL-TIME; 2-DEOXYMUGINEIC ACID; EXPRESSION PROFILE; TRANSPORTER FAMILY; REDUCTASE-ACTIVITY; METAL TRANSPORTER; ACTS DOWNSTREAM; TRANSLOCATION;
D O I
10.1111/nph.12577
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Although the genes involved in metal homeostasis have been investigated over the past few decades, many genes related to metal homeostasis remain uncharacterized, and a comprehensive analysis of the expression of these genes is required. In the present study, we investigated the spatial gene expression profile of iron (Fe)-deficient and cadmium (Cd)-stressed Oryza sativa (rice) using laser microdissection and micro-array analysis. Roots of Fe-deficient and Cd-stressed rice were separated into the vascular bundle, cortex, and epidermis plus exodermis. In addition, vascular bundles from new and old leaves at the lowest node, which are important for metal distribution, were analyzed separately. The spatial expression patterns were distinct in each tissue type. Fe deficiency and Cd stress also had significant effects on the transcriptomes, although these were less pronounced than the spatial effects. Genes encoding transporters involved in metal homeostasis, proteins associated with heavy metal detoxification, and phytohormone-related proteins were comprehensively investigated. Additionally, cis motifs involved in the regulation of these diverse expression changes in various tissue types were predicted. The spatial transcriptomes presented here provide novel insight into the molecular mechanisms of metal homeostasis.
引用
收藏
页码:781 / 794
页数:14
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