Dynamic effects of leflunomide on IL-1, IL-6, and TNF-α activity produced from peritoneal macrophages in adjuvant arthritis rats

被引：0
|
作者：
Li, WD [1 ]
Ran, GX [1 ]
Teng, HL [1 ]
Lin, ZB [1 ]
机构：
[1] Peking Univ, Hlth Sci Ctr, Dept Pharmacol, Beijing 100083, Peoples R China
来源：
ACTA PHARMACOLOGICA SINICA | 2002年 / 23卷 / 08期
关键词：
leflunomide; interleukin-1; interleukin-6; tumor necrosis factor; macrophages; adjuvant arthritis;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
AIM: To investigate the effects of leflunomide (LEF) on modulating interleukin-1 (IL-1), interleukin-6 (IL-6), and tumor necrosis factor- alpha (TNF-alpha) production induced by lipopolysaccharide (LPS) in peritoneal macrophages (PMphi) in adjuvant arthritis rats and elucidate the possible mechanisms of antiinflammatory and antirheumatoid effects of LEF. METHODS: Freund's complete adjuvant was injected in the hind footpad of rats to induce adjuvant arthritis (AA) rat model. The PMphi samples were taken at different time after medication. IL-1, IL-6, and TNF-alpha activities released from PMphi were measured by ELISA method or bioassay method. RESULTS: Production of IL-1, IL-6, and TNF-alpha was increased in the culture supernatant of PMphi in AA model rat. LEF could inhibit LPS-induced release of IL-1 and TNF-alpha from PMphi of the AA rats and the inhibitory effects were extremely rapid. LEF (10, 25 mg/kg) administrated for 21d could inhibit IL-6 release from PMphi in AA rats. CONCLUSION: The antiinflammatory mechanisms of LEF in AA rats might be related to inhibitory level of IL-1, IL-6, and TNF-alpha from PMphi in vivo.
引用
收藏
页码：752 / 756
页数：5
相关论文
共 50 条
  • [21] Role of TNF-α, IL-1, and IL-6 in experimental autoimmune bullous pemphigoid
    Zhao, M
    Carter, KE
    Diaz, LA
    Liu, Z
    JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2004, 122 (03) : A13 - A13
  • [22] Human osteoclasts secrete TNF-α and IL-6 in response to IL-1α stimulation
    Fuson, TR
    Galvin, RJS
    JOURNAL OF BONE AND MINERAL RESEARCH, 2002, 17 : S344 - S344
  • [23] The role of chicken IL-1β, IL-6 and TNF-α in the occurrence of amyloid arthropathy
    Sevimli, Alper
    Misirlioglu, Deniz
    Yagci, Artay
    Bulbul, Aziz
    Yilmaztepe, Arzu
    Altunbas, Korhan
    VETERINARY RESEARCH COMMUNICATIONS, 2008, 32 (07) : 499 - 508
  • [24] 肺炎患儿血清IL-1、IL-6、TNF-α的变化
    万军
    医药论坛杂志, 2003, (06) : 36 - 37
  • [25] Effect of Peritoneal Lavage on TNF-α, IL-1β, and IL-6 Levels and Mortality in Intra-Abdominal Infections
    Karakoc, Derya
    Sayek, Iskender
    INTERNATIONAL SURGERY, 2008, 93 (06) : 346 - 353
  • [26] The role of IL-1, IL-6 and TNF-α α in breast cancer development and progression
    Al-Qubati, Ahmed
    Rahmadi, Mahardian
    Widiandani, Tri
    Al-Maamari, Jamal
    Khotib, Junaidi
    PHARMACY EDUCATION, 2024, 24 (03): : 32 - 38
  • [27] IL-1β, IL-6 and TNF-α and outcomes of neonatal hypoxic ischemic encephalopathy
    Aly, H
    Khashaba, MT
    El-Ayouty, M
    El-Sayed, O
    Hasanein, BM
    BRAIN & DEVELOPMENT, 2006, 28 (03): : 178 - 182
  • [28] The role of chicken IL-1β, IL-6 and TNF-α in the occurrence of amyloid arthropathy
    Alper Sevimli
    Deniz Mısırlıoğlu
    Artay Yağcı
    Aziz Bülbül
    Arzu Yılmaztepe
    Korhan Altunbas
    Veterinary Research Communications, 2008, 32 : 499 - 508
  • [29] Cytokines IL-1β, IL-6, and TNF-α enhance in vitro growth of bacteria
    Meduri, GU
    Kanangat, S
    Stefan, J
    Tolley, E
    Schaberg, D
    AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 1999, 160 (03) : 961 - 967
  • [30] Effect of estrogen replacement therapy on serum cytokines (IL-1α, IL-6, TNF-α) in ovariectomized rats
    Rahnama, M
    Tomaszewski, T
    Swiatkowski, W
    BULLETIN OF THE VETERINARY INSTITUTE IN PULAWY, 2002, 46 (02) : 273 - 279
12345