Immunoadjuvant potential of Asparagus racemosus aqueous extract in experimental system

被引:80
作者
Gautam, M
Diwanay, S
Gairola, S
Shinde, Y
Patki, P
Patwardhan, B [1 ]
机构
[1] Univ Poona, Interdisciplinary Sch Hlth Sci, Pune 411007, Maharashtra, India
[2] Abasaheb Garware Coll, Dept Microbiol, Pune, Maharashtra, India
[3] India Res Fdn, Serum Inst, Pune 411042, Maharashtra, India
关键词
vaccine adjuvant; immunmodulator; immunostimulant; mouse protection test; ayurveda; Shatavari; Asparagus racemosus;
D O I
10.1016/j.jep.2003.12.023
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The immunoadjuvant potential of Asparagus racemosus (Willd.) Family (Liliaceae) aqueous root extract was evaluated in experimental animals immunized with diphtheria, tetanus, pertussis (DTP) vaccine. Immunostimulation was evaluated using serological and hematological parameters. Oral administration of test material at 100 mg/kg per day dose for 15 days resulted significant increase (P = 0.0052) in antibody titers to Bordtella pertussis as compared to untreated (control) animals. Immunized animals (treated and untreated) were challenged with B. pertussis 18323 strain and the animals were observed for 14 days. Results indicate that the treated animals did show significant increase in antibody titers as compared to untreated annuals after challenge (P = 0.002). Immunoprotection against intra-cerebral challenge of live B. pertussis cells was evaluated based on degree of sickness, paralysis and Subsequent death. Reduced mortality accompanied with overall improved health status was observed in treated animals after intra-cerebral challenge of B. pertussis indicating development of protective immune response. Present study indicates applications of test material as potential immunoadjuvant that also offers direct therapeutic benefits resulting in less morbidity and mortality. (C) 2004 Published by Elsevier Ireland Ltd.
引用
收藏
页码:251 / 255
页数:5
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