An amperometric biosensor for glucose detection from glucose oxidase immobilized in polyaniline-polyvinylsulfonate-potassium ferricyanide film

被引：21
|
作者：
Arslan, Fatma [1 ]
Beskan, Umut [2 ]
机构：
[1] Gazi Univ, Fac Sci, Dept Chem, Ankara, Turkey
[2] Gazi Univ, Inst Sci, Dept Chem, Ankara, Turkey
来源：
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY | 2014年 / 42卷 / 04期
关键词：
amperometric biosensor; ferricyanide; glucose; polyaniline; polyvinylsulphonate; POLYPYRROLE FILM; ELECTRODE; FERROCENE; MEDIATOR; SENSORS;
D O I：
10.3109/21691401.2013.812650
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
In this study, a novel amperometric glucose biosensor with immobilization of glucose oxidase on electrochemically polymerized polyaniline-polyvinylsulphonate-potassium ferricyanide (Pani-Pvs-Fc) films has been accomplished via the entrapment technique. Potassium ferricyanide was used as the mediator. Determination of glucose was carried out by the oxidation of potassium ferrocyanide at 0.3 V vs. Ag/AgCl. The effects of pH and temperature were investigated, and the optimum pH value was found to be 7.5. The storage stability and the operational stability of the enzyme electrode were also studied.
引用
收藏
页码：284 / 288
页数：5
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