SARS-CoV-2 variants of concern surveillance including Omicron using RT-PCR-based genotyping offers comparable performance to whole genome sequencing

被引：1

作者：

Vanoni, Simone ^{[1
]}

Matulevicius, Arnoldas ^{[1
]}

Avdiu, Besard ^{[1
]}

Scantamburlo, Giada ^{[1
]}

Ulekleiv, Camilla ^{[2
]}

Brzoska, Pius M. ^{[2
]}

Furtado, Manohar R. ^{[2
]}

Feenstra, Jelena D. M. ^{[2
]}

Rico, Alain ^{[2
]}

Gandhi, Manoj ^{[2
]}

Giacobazzi, Elisabetta ^{[3
]}

Masi, Elisa ^{[3
]}

Paulmichl, Markus ^{[4
]}

Nofziger, Charity ^{[1
]}

机构：

[1] PharmGenetix GmbH, Niederalm Anif, Austria

[2] Thermo Fisher Sci, South San Francisco, CA USA

[3] Azienda Sanit Alto Adige, Lab Microbiol & Virol, Bolzano, Italy

[4] Privatklin Maria Hilf GmbH, Dept Personalised Med, Klagenfurt, Austria

来源：

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY | 2022年 / 12卷

关键词：

SARS-CoV-2; COVID-19; variants of concern; omicron; delta; alpha; whole genome sequencing; mutation panel; RECEPTOR-BINDING DOMAIN; MUTATIONS;

D O I：

10.3389/fcimb.2022.960065

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Known SARS-CoV-2 variants of concern (VOCs) can be detected and differentiated using an RT-PCR-based genotyping approach, which offers quicker time to result, lower cost, higher flexibility, and use of the same laboratory instrumentation for detection of SARS-CoV-2 when compared with whole genome sequencing (WGS). In the current study, we demonstrate how we applied a genotyping approach for identification of all VOCs and that such technique can offer comparable performance to WGS for identification of known SARS-CoV-2 VOCs, including more recent strains, Omicron BA.1 and BA.2.

引用

页数：7

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