Characterization of 31 EST-derived microsatellite markers for the pearl oyster Pinctada martensii (Dunker)

被引：15
作者：
Shi, Yaohua [1 ,2 ,3 ]
Wang, Yan [1 ]
Hong, Kui [3 ]
Hou, Zhanhui [1 ]
Wang, Aimin [1 ]
Guo, Ximing [2 ]
机构：
[1] Hainan Univ, Ocean Coll, Hainan Key Lab Trop Hydrobiol Technol, Key Lab Trop Biol Resources,MOE, Haikou 570228, Peoples R China
[2] Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA
[3] CATAS, Inst Trop Biosci & Biotechnol, State Key Biotechnol Lab Trop Crops, Haikou 571101, Peoples R China
来源：
MOLECULAR ECOLOGY RESOURCES | 2009年 / 9卷 / 01期
基金：
国家高技术研究发展计划(863计划); 中国国家自然科学基金;
关键词：
EST; genome mapping; microsatellites; pearl oyster; Pinctada martensii (Dunker); population genetics;
D O I：
10.1111/j.1755-0998.2008.02424.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We developed and characterized 31 microsatellite markers from expressed sequence tags of Pinctada martensii (Dunker). The number of alleles per locus ranged from 4 to 18 as determined in 44 individuals from a wild population. The expected heterozygosity ranged from 0.4121 to 0.9436, while the observed heterozygosity ranged from 0.4054 to 0.7273. Most of the loci are in Hardy-Weinberg equilibrium. These markers should be useful for population genetics studies, parentage and genome mapping in this species.
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页码：177 / 179
页数：3
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