Characterization of 31 EST-derived microsatellite markers for the pearl oyster Pinctada martensii (Dunker)

被引：15

作者：

Shi, Yaohua ^{[1
,2
,3
]}

Wang, Yan ^{[1
]}

Hong, Kui ^{[3
]}

Hou, Zhanhui ^{[1
]}

Wang, Aimin ^{[1
]}

Guo, Ximing ^{[2
]}

机构：

[1] Hainan Univ, Ocean Coll, Hainan Key Lab Trop Hydrobiol Technol, Key Lab Trop Biol Resources,MOE, Haikou 570228, Peoples R China

[2] Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA

[3] CATAS, Inst Trop Biosci & Biotechnol, State Key Biotechnol Lab Trop Crops, Haikou 571101, Peoples R China

来源：

MOLECULAR ECOLOGY RESOURCES | 2009年 / 9卷 / 01期

基金：

中国国家自然科学基金; 国家高技术研究发展计划(863计划);

关键词：

EST; genome mapping; microsatellites; pearl oyster; Pinctada martensii (Dunker); population genetics;

D O I：

10.1111/j.1755-0998.2008.02424.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed and characterized 31 microsatellite markers from expressed sequence tags of Pinctada martensii (Dunker). The number of alleles per locus ranged from 4 to 18 as determined in 44 individuals from a wild population. The expected heterozygosity ranged from 0.4121 to 0.9436, while the observed heterozygosity ranged from 0.4054 to 0.7273. Most of the loci are in Hardy-Weinberg equilibrium. These markers should be useful for population genetics studies, parentage and genome mapping in this species.

引用

页码：177 / 179

页数：3

共 5 条

[1]

Hedgecock D, 2004, J SHELLFISH RES, V23, P379

[2] DNA marker technologies and their applications in aquaculture genetics [J].