Single Low-Dose Ionizing Radiation Induces Genotoxicity in Adult Zebrafish and its Non-Irradiated Progeny

被引：9

作者：

Lemos, J. ^{[1
,2
]}

Neuparth, T. ^{[3
]}

Trigo, M. ^{[4
]}

Costa, P. ^{[2
]}

Vieira, D. ^{[2
]}

Cunha, L. ^{[5
]}

Ponte, F. ^{[6
]}

Costa, P. S. ^{[6
]}

Metello, L. F. ^{[2
,5
]}

Carvalho, A. P. ^{[3
,4
]}

机构：

[1] Univ Porto, ICBAS Inst Biomed Sci, Rua Jorge Viterbo Ferreira 228, P-4050313 Oporto, Portugal

[2] Polytech Inst Porto ESTSP IPP, High Inst Allied Hlth Technol Porto, Dept Nucl Med, Rua Valente Perfeito 322, P-4400330 Vila Nova De Gaia, Portugal

[3] Univ Porto, CIIMAR CIMAR, Interdisciplinary Ctr Marine & Environm Res, Rua Bragas 289, P-4050123 Oporto, Portugal

[4] Univ Porto, Fac Sci, Dept Biol, Rua Campo Alegre, P-4169007 Oporto, Portugal

[5] IsoPor SA, POB 4028, P-4445 Ermesinde, Portugal

[6] Julio Teixeira SA, Radiotherapy Deptartment, Rua Arquitecto Cassiano Barbosa 6,F,Sala 26, P-4100009 Oporto, Portugal

来源：

BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY | 2017年 / 98卷 / 02期

关键词：

Zebrafish; Ionizing radiation; Genotoxicity; Comet assay; DETECTING DNA-DAMAGE; GAMMA-RADIATION; COMET ASSAY; MEDAKA FISH; MICRONUCLEUS ASSAY; EXPOSURE; MARINE; IRRADIATION; EXPRESSION; BIOMARKER;

D O I：

10.1007/s00128-016-2006-1

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

This study investigated to what extent a single exposure to low doses of ionizing radiation can induce genotoxic damage in irradiated adult zebrafish (Danio rerio) and its non-irradiated F1 progeny. Four groups of adult zebrafish were irradiated with a single dose of X-rays at 0 (control), 100, 500 and 1000 mGy, respectively, and couples of each group were allowed to reproduce following irradiation. Blood of parental fish and whole-body offspring were analysed by the comet assay for detection of DNA damage. The level of DNA damage in irradiated parental fish increased in a radiation dose-dependent manner at day 1 post-irradiation, but returned to the control level thereafter. The level of DNA damage in the progeny was directly correlated with the parental irradiation dose. Results highlight the genotoxic risk of a single exposure to low-dose ionizing radiation in irradiated individuals and also in its non-irradiated progeny.

引用

页码：190 / 195

页数：6

共 42 条

[1] Transgenerational changes in somatic and germ line genetic integrity of first-generation offspring derived from the DNA damaged sperm
Adiga, Satish Kumar
Upadhya, Dinesh
Kalthur, Guruprasad
Sadashiva, Satish Rao Bola
Kumar, Pratap
[J]. FERTILITY AND STERILITY, 2010, 93 (08) : 2486 - 2490
[2] Assessing the genotoxic potentials of arsenic in tilapia (Oreochromis mossambicus) using alkaline comet assay and micronucleus test
Ahmed, Md Kawser
Habibullah-Al-Mamun, Md
Hossain, M. Anwar
Arif, Mohammad
Parvin, Elora
Akter, Mosammat Salma
Khan, Mohammad Shahneawz
Islam, Md Monirul
[J]. CHEMOSPHERE, 2011, 84 (01) : 143 - 149
[3] Gamma radiation induced cell cycle perturbations and DNA damage in Catla Catla as measured by flow cytometry
Anbumani, S.
Mohankumar, Mary N.
[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2015, 113 : 18 - 22
[4] Gamma radiation induced micronuclei and erythrocyte cellular abnormalities in the fish Catla catla
Anbumani, S.
Mohankumar, Mary N.
[J]. AQUATIC TOXICOLOGY, 2012, 122 : 125 - 132
[5] Banu BS, 2001, FOOD CHEM TOXICOL, V39, P361, DOI 10.1016/S0278-6915(00)00141-1
[6] The offspring of irradiated parents, are they stable?
Barber, Ruth C.
Dubrova, Yuri E.
[J]. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2006, 598 (1-2) : 50 - 60
[7] Quantification of ionizing radiation-induced cell death in situ in a vertebrate embryo
Bladen, Catherine L.
Flowers, Melody A.
Miyake, Katsuya
Podolsky, Robert H.
Barrett, John T.
Kozlowski, David J.
Dynan, William S.
[J]. RADIATION RESEARCH, 2007, 168 (02) : 149 - 157
[8] Embryos of the zebrafish Danio rerio in studies of non-targeted effects of ionizing radiation
Choi, V. W. Y.
Yu, K. N.
[J]. CANCER LETTERS, 2015, 356 (01) : 91 - 104
[9] The comet assay: topical issues
Collins, Andrew R.
Oscoz, Amaia Azqueta
Brunborg, Gunnar
Gaivao, Isabel
Giovannelli, Lisa
Kruszewski, Marcin
Smith, Catherine C.
Stetina, Rudolf
[J]. MUTAGENESIS, 2008, 23 (03) : 143 - 151
[10] The comet assay: a heavenly method!
Collins, Andrew R.
[J]. MUTAGENESIS, 2015, 30 (01) : 1 - 4

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